دورية أكاديمية

Meta-Analysis of the QTLome of Fusarium Head Blight Resistance in Bread Wheat: Refining the Current Puzzle

التفاصيل البيبلوغرافية
العنوان: Meta-Analysis of the QTLome of Fusarium Head Blight Resistance in Bread Wheat: Refining the Current Puzzle
المؤلفون: Eduardo Venske, Railson Schreinert dos Santos, Daniel da Rosa Farias, Vianei Rother, Luciano Carlos da Maia, Camila Pegoraro, Antonio Costa de Oliveira
المصدر: Frontiers in Plant Science, Vol 10 (2019)
بيانات النشر: Frontiers Media S.A., 2019.
سنة النشر: 2019
المجموعة: LCC:Plant culture
مصطلحات موضوعية: molecular markers, meta-QTL, genetic architecture, genetic maps, genome, transcriptome, Plant culture, SB1-1110
الوصف: Background: Fusarium Head Blight (FHB) is a worldwide devastating disease of bread wheat (Triticum aestivum L.). Genetic resistance is the most effective way to control FHB and many QTL related to this trait have been mapped on the wheat genetic map. This information, however, must be refined to be more efficiently used in breeding programs and for the advance of the basic research. The objective of the present study was to in-depth analyze the QTLome of FHB resistance in bread wheat, further integrating genetic, genomic, and transcriptomic data, aiming to find candidate genes.Methods: An exhaustive bibliographic review on 76 scientific papers was carried out collecting information about QTL related to FHB resistance mapped on bread wheat. A dense genetic consensus map with 572,862 loci was generated for QTL projection. Meta-analysis could be performed on 323 QTL. Candidate gene mining was carried out within the most refined loci, containing genes that were cross-validated with publicly available transcriptional expression data of wheat under Fusarium infection. Most highlighted genes were investigated for protein evidence.Results: A total of 556 QTL were found in the literature, distributed on all sub-genomes and chromosomes of wheat. Meta-analysis generated 65 meta-QTL, and this refinement allows one to find markers more tightly linked to these regions. Candidate gene mining within the most refined meta-QTL, meta-QTL 1/chr. 3B, harvested 324 genes and transcriptional data cross-validated 10 of these genes, as responsive to FHB. One is of these genes encodes a Glycosiltransferase and the other encodes for a Cytochrome P450, and these such proteins have already been verified as being responsible for FHB resistance, but the remaining eight genes still have to be further studied, as promising loci for breeding.Conclusions: The QTLome of FHB resistance in wheat was successfully assembled and a refinement in terms of number and length of loci was obtained. The integration of the QTLome with genomic and transcriptomic data has allowed for the discovery of promising candidate genes for use in breeding programs.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1664-462X
Relation: https://www.frontiersin.org/article/10.3389/fpls.2019.00727/full; https://doaj.org/toc/1664-462X
DOI: 10.3389/fpls.2019.00727
URL الوصول: https://doaj.org/article/4ed55f7561264e418fbb269be1c4b979
رقم الأكسشن: edsdoj.4ed55f7561264e418fbb269be1c4b979
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:1664462X
DOI:10.3389/fpls.2019.00727