دورية أكاديمية
Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5
| العنوان: | Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5 |
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| المؤلفون: | Junyi Zhao, Jun Shi, Yun Shan, Manshu Yu, Xiaolin Zhu, Yilin Zhu, Li Liu, Meixiao Sheng |
| المصدر: | Cellular & Molecular Biology Letters, Vol 25, Iss 1, Pp 1-15 (2020) |
| بيانات النشر: | BMC, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Cytology |
| مصطلحات موضوعية: | Human peritoneal mesothelial cells (HPMCs), Mesothelial-mesenchymal transition (MMT), Reactive oxygen species (ROS), TGF-β/Smad, Nrf-2/HO-1, Cytology, QH573-671 |
| الوصف: | Abstract Background Peritoneal fibrosis (PF) is a frequent complication caused by peritoneal dialysis (PD). Peritoneal mesothelial cells (PMCs), the first barrier of the peritoneum, play an important role in maintaining structure and function in the peritoneum during PD. Mesothelial-mesenchymal transition (MMT) and oxidative stress of PMCs are two key processes of PF. Purpose To elucidate the efficacy and possible mechanism of asiaticoside inhibition of MMT and ROS generation in TGF-β1-induced PF in human peritoneal mesothelial cells (HPMCs). Methods MMT and ROS generation of HPMCs were induced by TGF-β1. To explain the anti-MMT and antioxidant role of asiaticoside, varied doses of asiaticoside, oxygen radical scavenger (NAC), TGF-β receptor kinase inhibitor (LY2109761) and Nrf2 inhibitor (ML385) were used separately. Immunoblots were used to detect the expression of signaling associated proteins. DCFH-DA was used to detect the generation of ROS. Transwell migration assay and wound healing assay were used to verify the capacity of asiaticoside to inhibit MMT. Immunofluorescence assay was performed to observe the subcellular translocation of Nrf2 and expression of HO-1. Results Asiaticoside inhibited TGF-β1-induced MMT and suppressed Smad signaling in a dose-dependent manner. Migration and invasion activities of HPMCs were decreased by asiaticoside. Asiaticoside decreased TGF-β1-induced ROS, especially in a high dose (150 μM) for 6 h. Furthermore, ML385 partly abolished the inhibitory effect of asiaticoside on MMT, ROS and p-Smad2/3. Conclusions Asiaticoside inhibited the TGF-β1-induced MMT and ROS via Nrf2 activation, thus protecting the peritoneal membrane and preventing PF. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1425-8153 1689-1392 |
| Relation: | http://link.springer.com/article/10.1186/s11658-020-00226-9; https://doaj.org/toc/1425-8153; https://doaj.org/toc/1689-1392 |
| DOI: | 10.1186/s11658-020-00226-9 |
| URL الوصول: | https://doaj.org/article/4edd97dd706d4c809ce7d875ae78ba6f |
| رقم الأكسشن: | edsdoj.4edd97dd706d4c809ce7d875ae78ba6f |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14258153 16891392 |
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| DOI: | 10.1186/s11658-020-00226-9 |