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Low-temperature preservation of sterlet reproductive cells (Acipenser ruthenus Linnaeus, 1758) by the acoustic-mechanical influence method

التفاصيل البيبلوغرافية
العنوان: Low-temperature preservation of sterlet reproductive cells (Acipenser ruthenus Linnaeus, 1758) by the acoustic-mechanical influence method
المؤلفون: Ponomareva Elena, Matrosov Andrey, Kovalenko Matvey, Rudoy Dmitry, Pakhomov Viktor
المصدر: E3S Web of Conferences, Vol 462, p 03058 (2023)
بيانات النشر: EDP Sciences, 2023.
سنة النشر: 2023
المجموعة: LCC:Environmental sciences
مصطلحات موضوعية: Environmental sciences, GE1-350
الوصف: To preserve reproductive cells of rare and endangered species of sturgeon fish using low-temperature cryopreservation methods, acoustic-mechanical impact with the use of piezoactuators was used. As a result of the research, the optimum parameters of signal frequency and time of impact on sturgeon cells were established, at which a high survival rate of sperm after defrosting was observed.Using mathematical modelling, the mechanism of acoustic-mechanical influence was studied, which consists in the wave and mechanical action created by the piezoactuator, which leads to acceleration of the process of diffusion of the cryoprotector inside the cells.For the first time a new methodological approach to low-temperature preservation of fish reproductive cells with the use of acoustic-mechanical impact has been proposed, which makes it possible to preserve genetic material with high survival rate and quality indicators.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
French
تدمد: 2267-1242
Relation: https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/99/e3sconf_afe23_03058.pdf; https://doaj.org/toc/2267-1242
DOI: 10.1051/e3sconf/202346203058
URL الوصول: https://doaj.org/article/4f32b0582c2c415892f28710d69ee386
رقم الأكسشن: edsdoj.4f32b0582c2c415892f28710d69ee386
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:22671242
DOI:10.1051/e3sconf/202346203058