دورية أكاديمية
miRNA-451 regulates rhesus choroid-retinal endothelial cell function and proteome profile
العنوان: | miRNA-451 regulates rhesus choroid-retinal endothelial cell function and proteome profile |
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المؤلفون: | Hong-Lian Wu, Yan Shao, Zhen-Na Chen, Hui Zhang, Xiao-Min Zhang, Xiao-Rong Li |
المصدر: | International Journal of Ophthalmology, Vol 15, Iss 6, Pp 894-904 (2022) |
بيانات النشر: | Press of International Journal of Ophthalmology (IJO PRESS), 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Ophthalmology |
مصطلحات موضوعية: | mirna-451, rf/6a, retinal endothelial cells, itraq quantitative proteomics, proteins, ptpn1, Ophthalmology, RE1-994 |
الوصف: | AIM: To evaluate the effect of miRNA-451 on rhesus macaque choroid-retinal endothelial (RF/6A) cell function and proteome profile. METHODS: The RF/6A cells were transfected with miRNA-451 mimic and inhibitor. The role of miRNA-451 on proliferation ability was evaluated by CCK-8 assay. Furthermore, iTRAQ quantitative proteomic analysis was applied to comprehensively illuminate the change of cellular proteins and biological function between different groups. RESULTS: In miRNA-451 overexpression group, cell proliferation of RF/6A decreased both at 24h and 48h; while in miRNA-451 inhibition group, on the contrary, RF/6A cell proliferation was increased at 48h. Based on iTRAQ quantitative proteomic analysis, 23 differentially expressed proteins (DEPs) were detected in the comparison of miRNA-451 mimic and mimic control-transfected RF/6A cells, and 30 DEPs were identified in the comparison of RF/6A cells transfected with miRNA-451 inhibitor and inhibitor control. DEPs such as GORASP2, KRT1, SLC7A2, RIC8A, DDX42, CAP1, PCBP2 might be closely related to the inhibitory effect of miRNA-451 on RF/6A cell proliferation, while PCYT1A, MGAT1, TUBB, MCU, SIL1, BID, MSH6 might account for the positive effect of miRNA-451 inhibitor on RF/6A cell growth. PTPN1, as the only protein exhibiting an opposite trend between miRNA-451 mimic and inhibitor-transfected cells, was most likely accountable for the inhibition of miRNA-451 mimic on RF/6A cell growth, and the promotion of miRNA-451 inhibitor on RF/6A cell proliferation. CONCLUSION: miRNA-451 overexpression can suppress the growth of RF/6A cells while knockdown of miRNA-451 can promote RF/6A cell viability. Among all DEPs, increased PTPN1 is most likely to account for the negative regulation of miRNA-451 on RF/6A proliferation. miRNA-451 can be a protective factor for neovascular disease of fundus via regulating choroid retinal endothelial cell function. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2222-3959 2227-4898 |
Relation: | http://ies.ijo.cn/en_publish/2022/6/20220606.pdf; https://doaj.org/toc/2222-3959; https://doaj.org/toc/2227-4898 |
DOI: | 10.18240/ijo.2022.06.06 |
URL الوصول: | https://doaj.org/article/4f7e9ffd49524d10ba3122e78c67e0c7 |
رقم الأكسشن: | edsdoj.4f7e9ffd49524d10ba3122e78c67e0c7 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 22223959 22274898 |
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DOI: | 10.18240/ijo.2022.06.06 |