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Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from Sulfolobus acidocaldarius

التفاصيل البيبلوغرافية
العنوان: Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from Sulfolobus acidocaldarius
المؤلفون: Konrad H. Engelhardt, Shashank Reddy Pinnapireddy, Elias Baghdan, Jarmila Jedelská, Udo Bakowsky
المصدر: Archaea, Vol 2017 (2017)
بيانات النشر: Wiley, 2017.
سنة النشر: 2017
المجموعة: LCC:Microbiology
مصطلحات موضوعية: Microbiology, QR1-502
الوصف: Lipid vectors are commonly used to facilitate the transfer of nucleic acids into mammalian cells. In this study, two fractions of tetraether lipids from the archaea Sulfolobus acidocaldarius were extracted and purified using different methods. The purified lipid fractions polar lipid fraction E (PLFE) and hydrolysed glycerol-dialkyl-nonitol tetraether (hGDNT) differ in their structures, charge, size, and miscibility from conventional lipids. Liposomes were prepared by mixing tetraether lipids with cholesterol (CH) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) resulting in stable vectors for gene delivery. Lipoplexes were prepared by complexation of liposomes with a luciferase expressing plasmid (pCMV-luc) at certain nitrogen-to-phosphorus (N/P) ratios and optimised for the transient transfection of ovarian adenocarcinoma cells (SK-OV-3). Complexation efficacy was investigated by gel-red fluorescence assay. Biophysical properties, like size, surface charge, and morphology, were investigated by differential light scattering (DLS), atomic force microscopy (AFM), and scanning electron microscopy (Cryo-SEM), respectively, revealing structural differences between liposomes and lipoplexes. A range of stable transfecting agents containing tetraether lipids were obtained by incorporating 5 mol% of tetraether lipids. Lipoplexes showed a decrease in free gel-red with increasing N/P ratios indicating efficient incorporation of plasmid DNA (pDNA) and remarkable stability. Transfection experiments of the lipoplexes revealed successful and superior transfection of SK-OV-3 cell line compared to the commercially available DOTAP and branched polyethyleneimine (25 kDa bPEI).
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1472-3646
1472-3654
Relation: https://doaj.org/toc/1472-3646; https://doaj.org/toc/1472-3654
DOI: 10.1155/2017/8047149
URL الوصول: https://doaj.org/article/d4f9efb757744bc6933df605dc7dfaea
رقم الأكسشن: edsdoj.4f9efb757744bc6933df605dc7dfaea
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14723646
14723654
DOI:10.1155/2017/8047149