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Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles

التفاصيل البيبلوغرافية
العنوان: Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles
المؤلفون: Byoung-cheun Lee, Cuong N. Duong, Jungkon Kim, Suejin Kim, Ig-chun Eom, Pilje Kim
المصدر: Applied Sciences, Vol 10, Iss 11, p 3796 (2020)
بيانات النشر: MDPI AG, 2020.
سنة النشر: 2020
المجموعة: LCC:Technology
LCC:Engineering (General). Civil engineering (General)
LCC:Biology (General)
LCC:Physics
LCC:Chemistry
مصطلحات موضوعية: nanoparticles, estrogen receptor, Japanese medaka, receptor binding assay, yeast, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999
الوصف: In order to investigate the effects of metallic nanoparticles (NPs) on the performance of in vitro bioassay, zinc oxide NP (ZnO NP), aluminum oxide NP (Al2O3 NP), bare silver NP (Ag NP), and Ag NP capped with citrate (Agcit NP) were evaluated with yeast (Saccharomyces cerevisiae Y190) two-hybrid system (YES assay), carrying Japanese medaka estrogen receptors (mERs) in the presence of 17β-estradiol (E2, 10−6 M), a reference chemical for estrogenic activity. The distribution of NPs in the yeast was also examined by field-emission transmission electron microscopy (FE-TEM). The results show that TEM analysis revealed that NPs were present inside the yeast and accumulated deep inside the cell organelles, suggesting that cell death was caused by NPs. However, despite no significant change of mortality, the E2 estrogenic activities in yeast exposed to ZnO NP and Al2O3 NP were dose-dependently reduced. For Ag NP and Agcit NP, such phenomenon observed in the exposure of ZnO NP and Al2O3 NP did not occur. From the observations, we found that ZnO NP and Al2O3 NP in the environmental media could result in underestimated estrogenicity of endocrine-disrupting compounds when evaluated by YES assay.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2076-3417
Relation: https://www.mdpi.com/2076-3417/10/11/3796; https://doaj.org/toc/2076-3417
DOI: 10.3390/app10113796
URL الوصول: https://doaj.org/article/500c91f3993d4d3d871841a3b8b10027
رقم الأكسشن: edsdoj.500c91f3993d4d3d871841a3b8b10027
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20763417
DOI:10.3390/app10113796