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Cytoglobin inhibits non-thermal plasma-induced apoptosis in melanoma cells through regulation of the NRF2-mediated antioxidant response

التفاصيل البيبلوغرافية
العنوان: Cytoglobin inhibits non-thermal plasma-induced apoptosis in melanoma cells through regulation of the NRF2-mediated antioxidant response
المؤلفون: Joey De Backer, Abraham Lin, Wim Vanden Berghe, Annemie Bogaerts, David Hoogewijs
المصدر: Redox Biology, Vol 55, Iss , Pp 102399- (2022)
بيانات النشر: Elsevier, 2022.
سنة النشر: 2022
المجموعة: LCC:Medicine (General)
LCC:Biology (General)
مصطلحات موضوعية: Melanoma, Cytoglobin, Non-thermal plasma, ROS, Apoptosis, Medicine (General), R5-920, Biology (General), QH301-705.5
الوصف: Melanoma arises from pigment-producing cells called melanocytes located in the basal layers of the epidermis of the skin. Cytoglobin (CYGB) is a ubiquitously expressed hexacoordinated globin that is highly enriched in melanocytes and frequently downregulated during melanomagenesis. Previously, we showed that non-thermal plasma (NTP)-produced reactive oxygen and nitrogen species (RONS) lead to the formation of an intramolecular disulfide bridge that would allow CYGB to function as a redox-sensitive protein. Here, we investigate the cytotoxic effect of indirect NTP treatment in two melanoma cell lines with divergent endogenous CYGB expression levels, and we explore the role of CYGB in determining treatment outcome. Our findings are consistent with previous studies supporting that NTP cytotoxicity is mediated through the production of RONS and leads to apoptotic cell death in melanoma cells. Furthermore, we show that NTP-treated solutions elicit an antioxidant response through the activation of nuclear factor erythroid 2–related factor 2 (NRF2). The knockdown and overexpression of CYGB respectively sensitizes and protects melanoma cells from RONS-induced apoptotic cell death. The presence of CYGB enhances heme-oxygenase 1 (HO-1) and NRF2 protein expression levels, whereas the absence impairs their expression. Moreover, analysis of the CYGB-dependent transcriptome demonstrates the tumor suppressor long non-coding RNA maternally expressed 3 (MEG3) as a hitherto undescribed link between CYGB and NRF2. Thus, the presence of CYGB, at least in melanoma cells, seems to play a central role in determining the therapeutic outcome of RONS-inducing anticancer therapies, like NTP-treated solutions, possessing both tumor-suppressive and oncogenic features. Hence, CYGB expression could be of interest either as a biomarker or as a candidate for future targeted therapies in melanoma.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2213-2317
Relation: http://www.sciencedirect.com/science/article/pii/S2213231722001719; https://doaj.org/toc/2213-2317
DOI: 10.1016/j.redox.2022.102399
URL الوصول: https://doaj.org/article/50232330a25d4eb9af0e6a59821c5130
رقم الأكسشن: edsdoj.50232330a25d4eb9af0e6a59821c5130
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:22132317
DOI:10.1016/j.redox.2022.102399