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Droplet digital PCR analysis of CDH13 methylation status in Slovak women with invasive ductal breast cancer

التفاصيل البيبلوغرافية
العنوان: Droplet digital PCR analysis of CDH13 methylation status in Slovak women with invasive ductal breast cancer
المؤلفون: Ivana Baranová, Marek Samec, Dana Dvorská, Igor Šťastný, Katarína Janíková, Ivana Kašubová, Andrea Hornáková, Eva Lukáčová, Andrea Kapinová, Kamil Biringer, Erika Halašová, Zuzana Danková
المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)
بيانات النشر: Nature Portfolio, 2024.
سنة النشر: 2024
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: CDH13, Droplet digital PCR, CpG methylation, MS-MLPA, Medicine, Science
الوصف: Abstract Identifying novel epigenetic biomarkers is a promising way to improve the clinical management of patients with breast cancer. Our study aimed to determine the methylation pattern of 25 tumor suppressor genes (TSG) and select the best methylation biomarker associated with clinicopathological features in the cohort of Slovak patients diagnosed with invasive ductal carcinoma (IDC). Overall, 166 formalin-fixed, paraffin-embedded (FFPE) tissues obtained from patients with IDC were included in the study. The methylation status of the promoter regions of 25 TSG was analyzed using semiquantitative methylation-specific MLPA (MS-MLPA). We identified CDH13 as the most frequently methylated gene in our cohort of patients. Further analysis by ddPCR confirmed an increased level of methylation in the promoter region of CDH13. A significant difference in CDH13 methylation levels was observed between IDC molecular subtypes LUM A versus HER2 (P = 0.0116) and HER2 versus TNBC (P = 0.0234). In addition, significantly higher methylation was detected in HER2+ versus HER2- tumors (P = 0.0004) and PR− versus PR+ tumors (P = 0.0421). Our results provide evidence that alteration in CDH13 methylation is associated with clinicopathological features in the cohort of Slovak patients with IDC. In addition, using ddPCR as a methylation-sensitive method represents a promising approach characterized by higher precision and technical simplicity to measure the methylation of target CpGs in CDH13 compared to other conventional methods such as MS-MLPA.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
Relation: https://doaj.org/toc/2045-2322
DOI: 10.1038/s41598-024-65580-6
URL الوصول: https://doaj.org/article/5091c256c8654dcf88a3b2049f72fddc
رقم الأكسشن: edsdoj.5091c256c8654dcf88a3b2049f72fddc
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-024-65580-6