دورية أكاديمية
Sarcocystis bovifelis in Raw Hamburgers Marketed in Hamadan City, Western Iran
العنوان: | Sarcocystis bovifelis in Raw Hamburgers Marketed in Hamadan City, Western Iran |
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المؤلفون: | Fariba Jafari, Seyed Mousa Motavallihaghi, Mehran Bakhtiari, Amir Hossein Maghsood, Faeze Foroughi-Parvar |
المصدر: | Iranian Journal of Parasitology, Vol 17, Iss 1 (2022) |
بيانات النشر: | Tehran University of Medical Sciences, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Infectious and parasitic diseases |
مصطلحات موضوعية: | Food microbiology, Sarcocystis spp, Sarcocystosis, Beef burger, Infectious and parasitic diseases, RC109-216 |
الوصف: | Background: We aimed to evaluate Sarcocystis contamination in conventional and industrial raw beef burger samples from butcheries and retail stores in Hamadan, western Iran. Methods: Overall, 80 samples including 30 conventional and 50 industrial hamburgers were randomly obtained from different butcheries and supermarkets. All specimens were studied by digestion method following microscopic examination. Samples` genomic ribosomal DNA were amplified and nucleotide sequences were analyzed by BLAST for comparison with the sequences in the gene bank of the NCBI. Results: Sarcocystis bradyzoites were detected in 46 of 80 (57.6%) samples. Positive specimens were included as 46 (57.6%) and 30 (37.5%) by digestion and molecular method, respectively. Differences between two studied (digestion and molecular) methods was statistically significant (P=0.00). Twenty-six (86.5 %) of 30 conventional beef burgers and 20 (40%) of 50 industrial burgers were positive for Sarcocystis sp. by digestion method. There was a significant difference between Sarcocystis infested conventional and industrial beef burgers (P=0.01). Conclusion: The parasitic contamination of beef burgers implied a high level of infection in cattle. Felids as the definitive hosts for S. bovifelis urged on the improvement of the hygienic conditions of keeping and feeding livestock in order to reduce the infection. Molecular techniques confirm species in meat products with high sensitivity and distinguish it from human species. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1735-7020 2008-238X |
Relation: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3428; https://doaj.org/toc/1735-7020; https://doaj.org/toc/2008-238X |
DOI: | 10.18502/ijpa.v17i1.9014 |
URL الوصول: | https://doaj.org/article/50935538b2464a339a1d769464078785 |
رقم الأكسشن: | edsdoj.50935538b2464a339a1d769464078785 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 17357020 2008238X |
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DOI: | 10.18502/ijpa.v17i1.9014 |