دورية أكاديمية

Sarcocystis bovifelis in Raw Hamburgers Marketed in Hamadan City, Western Iran

التفاصيل البيبلوغرافية
العنوان: Sarcocystis bovifelis in Raw Hamburgers Marketed in Hamadan City, Western Iran
المؤلفون: Fariba Jafari, Seyed Mousa Motavallihaghi, Mehran Bakhtiari, Amir Hossein Maghsood, Faeze Foroughi-Parvar
المصدر: Iranian Journal of Parasitology, Vol 17, Iss 1 (2022)
بيانات النشر: Tehran University of Medical Sciences, 2022.
سنة النشر: 2022
المجموعة: LCC:Infectious and parasitic diseases
مصطلحات موضوعية: Food microbiology, Sarcocystis spp, Sarcocystosis, Beef burger, Infectious and parasitic diseases, RC109-216
الوصف: Background: We aimed to evaluate Sarcocystis contamination in conventional and industrial raw beef burger samples from butcheries and retail stores in Hamadan, western Iran. Methods: Overall, 80 samples including 30 conventional and 50 industrial hamburgers were randomly obtained from different butcheries and supermarkets. All specimens were studied by digestion method following microscopic examination. Samples` genomic ribosomal DNA were amplified and nucleotide sequences were analyzed by BLAST for comparison with the sequences in the gene bank of the NCBI. Results: Sarcocystis bradyzoites were detected in 46 of 80 (57.6%) samples. Positive specimens were included as 46 (57.6%) and 30 (37.5%) by digestion and molecular method, respectively. Differences between two studied (digestion and molecular) methods was statistically significant (P=0.00). Twenty-six (86.5 %) of 30 conventional beef burgers and 20 (40%) of 50 industrial burgers were positive for Sarcocystis sp. by digestion method. There was a significant difference between Sarcocystis infested conventional and industrial beef burgers (P=0.01). Conclusion: The parasitic contamination of beef burgers implied a high level of infection in cattle. Felids as the definitive hosts for S. bovifelis urged on the improvement of the hygienic conditions of keeping and feeding livestock in order to reduce the infection. Molecular techniques confirm species in meat products with high sensitivity and distinguish it from human species.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1735-7020
2008-238X
Relation: https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3428; https://doaj.org/toc/1735-7020; https://doaj.org/toc/2008-238X
DOI: 10.18502/ijpa.v17i1.9014
URL الوصول: https://doaj.org/article/50935538b2464a339a1d769464078785
رقم الأكسشن: edsdoj.50935538b2464a339a1d769464078785
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:17357020
2008238X
DOI:10.18502/ijpa.v17i1.9014