دورية أكاديمية
Role of aggregate-forming pilus (AFP) in adherence and colonization of both intestinal and urinary tracts
| العنوان: | Role of aggregate-forming pilus (AFP) in adherence and colonization of both intestinal and urinary tracts |
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| المؤلفون: | Paulo A. Schüroff, Cecilia M. Abe, Jonatas W. Silva, Cidéli de Paula Coelho, Fernanda B. Andrade, Rodrigo T. Hernandes, Ulrich Dobrindt, Tânia A.T. Gomes, Waldir P. Elias |
| المصدر: | Virulence, Vol 13, Iss 1, Pp 1423-1433 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Hybrid-pathogenicE. coli, aggregate-forming pilus, AFP, urinary tract infection, intestinal colonization, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Hybrid-pathogenic Escherichia coli represent an important group of strains associated with intestinal and extraintestinal infections. Recently, we described strain UPEC-46, a uropathogenic/enteroaggregative E. coli (UPEC/EAEC) strain presenting the aggregative adherence (AA) pattern on bladder and colorectal epithelial cells mediated by aggregate-forming pili (AFP). However, the role of AFP and other uninvestigated putative fimbriae operons in UPEC-46 pathogenesis remains unclear. Thus, this study evaluated the involvement of AFP and other adhesins in uropathogenicity and intestinal colonization using different in vitro and in vivo models. The strain UPEC-46 was able to adhere and invade intestinal and urinary cell lines. A library of transposon mutants also identified the involvement of type I fimbriae (TIF) in the adherence to HeLa cells, in addition to colorectal and bladder cell lines. The streptomycin-treated mouse in vivo model also showed an increased number of bacterial counts in the colon in the presence of AFP and TIF. In the mouse model of ascending urinary tract infection (UTI), AFP was more associated with kidney colonization, while TIF appears to mediate bladder colonization. Results observed in in vivo experiments were also confirmed by electron microscopy (EM) analyses. In summary, the in vitro and in vivo analyses show a synergistic role of AFP and TIF in the adherence and colonization of intestinal and urinary epithelia. Therefore, we propose that hybrid E. coli strains carrying AFP and TIF could potentially cause intestinal and urinary tract infections in the same patient. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 21505594 2150-5608 2150-5594 |
| Relation: | https://doaj.org/toc/2150-5594; https://doaj.org/toc/2150-5608 |
| DOI: | 10.1080/21505594.2022.2112818 |
| URL الوصول: | https://doaj.org/article/509751498e2a496291a04991d52887c3 |
| رقم الأكسشن: | edsdoj.509751498e2a496291a04991d52887c3 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 21505594 21505608 |
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| DOI: | 10.1080/21505594.2022.2112818 |