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Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung.

التفاصيل البيبلوغرافية
العنوان: Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung.
المؤلفون: Katrien C De Grove, Sharen Provoost, Fien M Verhamme, Ken R Bracke, Guy F Joos, Tania Maes, Guy G Brusselle
المصدر: PLoS ONE, Vol 11, Iss 1, p e0145961 (2016)
بيانات النشر: Public Library of Science (PLoS), 2016.
سنة النشر: 2016
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Innate lymphoid cells (ILC) are a new family of innate immune cells that have emerged as important regulators of tissue homeostasis and inflammation. However, limited data are available concerning the relative abundance and characteristics of ILC in the human lung.The aim of this study was to characterize and enumerate the different ILC subsets in human lung by multi-color flow cytometry.Within the CD45+ Lin- CD127+ pulmonary ILC population, we identified group 1 (ILC1), group 2 (ILC2) and group 3 (ILC3) innate lymphoid cells using specific surface markers (i.e. IL12Rβ2, CRTH2 and CD117 respectively) and key transcription factors (i.e. T-bet, GATA-3 and RORγT respectively). Based on the presence of NKp44, ILC3 were further subdivided in natural cytotoxicity receptor (NCR)+ and NCR- ILC3. In addition, we demonstrated the production of signature cytokines IFN-γ, IL-5, IL-17A, IL-22 and GM-CSF in the pulmonary ILC population. Interestingly, we observed a tendency to a higher frequency of NCR- ILC3 in lungs of patients with chronic obstructive pulmonary disease (COPD) compared with controls.We show that the three main ILC subsets are present in human lung. Importantly, the relative abundance of ILC subsets tended to change in COPD patients in comparison to control individuals.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC4699688?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0145961
URL الوصول: https://doaj.org/article/511789b37a194f7688c2346249d27571
رقم الأكسشن: edsdoj.511789b37a194f7688c2346249d27571
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0145961