دورية أكاديمية
Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD
| العنوان: | Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD |
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| المؤلفون: | Ji Hyun Jeong, Seon Yu Lee, Bo Na Kim, Guk Yeo Lee, Seong Ho Ham |
| المصدر: | Journal of Analytical Methods in Chemistry, Vol 2018 (2018) |
| بيانات النشر: | Wiley, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Analytical chemistry |
| مصطلحات موضوعية: | Analytical chemistry, QD71-142 |
| الوصف: | So-Cheong-Ryong-Tang, which is a standardized Korean medicine of the National Health Insurance, is a traditional prescription for the treatment of allergic rhinitis, bronchitis, and bronchial asthma. Simultaneous analysis and development of SCRT is essential for its stability, efficacy, and risk management. In this study, a simple, reliable, and accurate method using ultrahigh-performance liquid chromatography (UPLC) fingerprinting with a diode array detector (DAD) was developed for the simultaneous analysis. The chromatographic separation of the analytes was performed by an ACQUITY UPLC BEH C18 column (1.7 μM, 2.1 × 100 mm, Waters) with a mobile phase of water containing 0.01% (v/v) phosphoric acid and acetonitrile containing 0.01% (v/v) phosphoric acid. The flow rate and detection wavelength were set at 0.4 mL/min and 215, 230, 254, and 280 nm. All calibration curves of the thirteen components showed good linearity (R2 > 0.999). The limit of detection and limit of quantification ranged 0.001–0.360 and 0.004–1.200 µg/mL, respectively. The relative standard deviation (RSD) of intra- and interday was less than 2.60%, and the recoveries were within the range 76.08–103.79% with an RSD value of 0.03–1.50%. The results showed that the developed method was simple, reliable, accurate, sensitive, and precise for the quantification of bioactive components of SCRT. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2090-8865 2090-8873 |
| Relation: | https://doaj.org/toc/2090-8865; https://doaj.org/toc/2090-8873 |
| DOI: | 10.1155/2018/2875681 |
| URL الوصول: | https://doaj.org/article/51e79565475a4c1889e7eed612c95019 |
| رقم الأكسشن: | edsdoj.51e79565475a4c1889e7eed612c95019 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20908865 20908873 |
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| DOI: | 10.1155/2018/2875681 |