دورية أكاديمية
Nanosecond pulsed electric fields enhance mesenchymal stem cells differentiation via DNMT1-regulated OCT4/NANOG gene expression
العنوان: | Nanosecond pulsed electric fields enhance mesenchymal stem cells differentiation via DNMT1-regulated OCT4/NANOG gene expression |
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المؤلفون: | Kejia Li, Tong Ning, Hao Wang, Yangzi Jiang, Jue Zhang, Zigang Ge |
المصدر: | Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-14 (2020) |
بيانات النشر: | BMC, 2020. |
سنة النشر: | 2020 |
المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
مصطلحات موضوعية: | Mesenchymal stem cells, Nanosecond pulsed electric fields, Demethylation, DNMT1, Biophysical stimulation, Stem cell differentiation, Medicine (General), R5-920, Biochemistry, QD415-436 |
الوصف: | Abstract Background Multiple strategies have been proposed to promote the differentiation potential of mesenchymal stem cells (MSCs), which is the fundamental property in tissue formation and regeneration. However, these strategies are relatively inefficient that limit the application. In this study, we reported a novel and efficient strategy, nanosecond pulsed electric fields (nsPEFs) stimulation, which can enhance the trilineage differentiation potential of MSCs, and further explained the mechanism behind. Methods We used histological staining to screen out the nsPEFs parameters that promoted the trilineage differentiation potential of MSCs, and further proved the effect of nsPEFs by detecting the functional genes. In order to explore the corresponding mechanism, we examined the expression of pluripotency genes and the methylation status of their promoters. Finally, we targeted the DNA methyltransferase which was affected by nsPEFs. Results The trilineage differentiation of bone marrow-derived MSCs was significantly enhanced in vitro by simply pre-treating with 5 pulses of nsPEFs stimulation (energy levels as 10 ns, 20 kV/cm; 100 ns, 10 kV/cm), due to that the nsPEFs demethylated the promoters of stem cell pluripotency genes OCT4 and NANOG through instantaneous downregulation of DNA methylation transferase 1 (DNMT1), thereby increasing the expression of OCT4 and NANOG for up to 3 days, and created a treatment window period of stem cells. Conclusions In summary, nsPEFs can enhance MSCs differentiation via the epigenetic regulation and could be a safe and effective strategy for future stem cell application. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1757-6512 |
Relation: | http://link.springer.com/article/10.1186/s13287-020-01821-5; https://doaj.org/toc/1757-6512 |
DOI: | 10.1186/s13287-020-01821-5 |
URL الوصول: | https://doaj.org/article/ca520fa6d1db41b08bb3df87f40d1d13 |
رقم الأكسشن: | edsdoj.520fa6d1db41b08bb3df87f40d1d13 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 17576512 |
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DOI: | 10.1186/s13287-020-01821-5 |