To investigate the effect of zein addition on rheological properties of buckwheat-based gluten-free dough, atmosphere puffed tartary buckwheat flour was mixed with common buckwheat flour at a ratio of 1:1 to make a mxied base flour (MBF). Zein was plasticized with 90% ethanol solution, and then 0%, 5%, 10%, 15%, and 20% (MBF based) of the plasticized zein was mixed with MBF and water, respectively, to make a buckwheat-based gluten-free dough (BBGD) with gradient content of zein. The static rheological properties of dough (texture properties, tensile properties and stress relaxation properties), dynamic rheological properties, optical properties (chromaticity and reflectivity) and microstructure were measured. Results showed that, with the increasing of zein content from 0% to 20%, the hardness of BBGD decreased from 482.38 g to 346.60 g, while elasticity, tensile distance, and tensile strength increased from 0.21 to 0.29, 15.44 to 38.16 mm, and 13.10 to 72.04 g, respectively. The residual stress, viscoelastic coefficient and relaxation time increased significantly compared with the control (P