دورية أكاديمية
Tachykinin acts upstream of autocrine Hedgehog signaling during nociceptive sensitization in Drosophila
| العنوان: | Tachykinin acts upstream of autocrine Hedgehog signaling during nociceptive sensitization in Drosophila |
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| المؤلفون: | Seol Hee Im, Kendra Takle, Juyeon Jo, Daniel T Babcock, Zhiguo Ma, Yang Xiang, Michael J Galko |
| المصدر: | eLife, Vol 4 (2015) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | nociceptive sensitization, Substance P, Hedgehog, electrophysiology, Trp channels, neuropeptide, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Pain signaling in vertebrates is modulated by neuropeptides like Substance P (SP). To determine whether such modulation is conserved and potentially uncover novel interactions between nociceptive signaling pathways we examined SP/Tachykinin signaling in a Drosophila model of tissue damage-induced nociceptive hypersensitivity. Tissue-specific knockdowns and genetic mutant analyses revealed that both Tachykinin and Tachykinin-like receptor (DTKR99D) are required for damage-induced thermal nociceptive sensitization. Electrophysiological recording showed that DTKR99D is required in nociceptive sensory neurons for temperature-dependent increases in firing frequency upon tissue damage. DTKR overexpression caused both behavioral and electrophysiological thermal nociceptive hypersensitivity. Hedgehog, another key regulator of nociceptive sensitization, was produced by nociceptive sensory neurons following tissue damage. Surprisingly, genetic epistasis analysis revealed that DTKR function was upstream of Hedgehog-dependent sensitization in nociceptive sensory neurons. Our results highlight a conserved role for Tachykinin signaling in regulating nociception and the power of Drosophila for genetic dissection of nociception. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/10735; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.10735 |
| URL الوصول: | https://doaj.org/article/522f8d3c8ef9484ba0c3cc37a756b006 |
| رقم الأكسشن: | edsdoj.522f8d3c8ef9484ba0c3cc37a756b006 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.10735 |