دورية أكاديمية
Rapid detection of human blood in triatomines (kissing bugs) utilizing a lateral flow immunochromatographic assay - A pilot study
| العنوان: | Rapid detection of human blood in triatomines (kissing bugs) utilizing a lateral flow immunochromatographic assay - A pilot study |
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| المؤلفون: | Norman L Beatty, Nicole Behrens-Bradley, Maria Love, Finn McCants, Shannon Smith, Justin O Schmidt, Sarah A Hamer, Patricia L Dorn, Nafees Ahmad, Stephen A Klotz |
| المصدر: | Memorias do Instituto Oswaldo Cruz, Vol 114, Iss 0 |
| بيانات النشر: | Fundação Oswaldo Cruz (FIOCRUZ). |
| المجموعة: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | triatomine, kissing bug, human blood detection, blood sources, Chagas disease, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| الوصف: | BACKGROUND DNA- and proteomics-based techniques are currently used to identify a triatomine human blood meal. These methods are time consuming, require access to laboratories with sophisticated equipment, and trained personnel. OBJECTIVES We tested a rapid and specific immunochromatographic assay (that detects human blood in forensic samples) to determine if human blood was present in triatomines and their fecal excreta. METHODS We fed Triatoma rubida human blood (positive control) or mouse blood (negative control) and performed the assay on the abdominal contents and fecal excreta. Triatomine field specimens collected in and around human habitations and excreta were also tested. FINDINGS The assay was positive in triatomines fed human blood (N = 5/5) and fecal excreta from bugs known to have ingested human blood (N = 5/5). Bugs feeding on mice (N = 15/15) and their fecal excreta (N = 8/8) were negative for human blood. Human blood was detected in 47% (N = 23/49) triatomines, representing six different species, collected in the field. MAIN CONCLUSIONS The pilot study shows that this rapid and specific test may have applications in triatomine research. Further study is needed to determine the sensitivity of this assay compared to other well-established techniques, such as DNA- and proteomics-based methodologies and the assay’s application in the field. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-8060 0074-0276 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100331&lng=en&tlng=en; https://doaj.org/toc/1678-8060 |
| DOI: | 10.1590/0074-02760190047 |
| URL الوصول: | https://doaj.org/article/524bf7939bc8487580e81027c953942f |
| رقم الأكسشن: | edsdoj.524bf7939bc8487580e81027c953942f |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 16788060 00740276 |
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| DOI: | 10.1590/0074-02760190047 |