دورية أكاديمية
Role of H2O2 in the oxidative effects of zinc exposure in human airway epithelial cells
| العنوان: | Role of H2O2 in the oxidative effects of zinc exposure in human airway epithelial cells |
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| المؤلفون: | Phillip A. Wages, Robert Silbajoris, Adam Speen, Luisa Brighton, Andres Henriquez, Haiyan Tong, Philip A. Bromberg, Steven O. Simmons, James M. Samet |
| المصدر: | Redox Biology, Vol 3, Iss C, Pp 47-55 (2014) |
| بيانات النشر: | Elsevier, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | Zinc, H2O2, Live cell imaging, Mitochondria, Catalase, Genetically encoded redox probes, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Human exposure to particulate matter (PM) is a global environmental health concern. Zinc (Zn2+) is a ubiquitous respiratory toxicant that has been associated with PM health effects. However, the molecular mechanism of Zn2+ toxicity is not fully understood. H2O2 and Zn2+ have been shown to mediate signaling leading to adverse cellular responses in the lung and we have previously demonstrated Zn2+ to cause cellular H2O2 production. To determine the role of Zn2+-induced H2O2 production in the human airway epithelial cell response to Zn2+ exposure. BEAS-2B cells expressing the redox-sensitive fluorogenic sensors HyPer (H2O2) or roGFP2 (EGSH) in the cytosol or mitochondria were exposed to 50 µM Zn2+ for 5 min in the presence of 1 µM of the zinc ionophore pyrithione. Intracellular H2O2 levels were modulated using catalase expression either targeted to the cytosol or ectopically to the mitochondria. HO-1 mRNA expression was measured as a downstream marker of response to oxidative stress induced by Zn2+ exposure. Both cytosolic catalase overexpression and ectopic catalase expression in mitochondria were effective in ablating Zn2+-induced elevations in H2O2. Compartment-directed catalase expression blunted Zn2+-induced elevations in cytosolic EGSH and the increased expression of HO-1 mRNA levels. Zn2+ leads to multiple oxidative effects that are exerted through H2O2-dependent and independent mechanisms. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2213-2317 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2213231714001116; https://doaj.org/toc/2213-2317 |
| DOI: | 10.1016/j.redox.2014.10.005 |
| URL الوصول: | https://doaj.org/article/5267f6d5c3674fb59fe29b71a7d8fcbe |
| رقم الأكسشن: | edsdoj.5267f6d5c3674fb59fe29b71a7d8fcbe |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22132317 |
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| DOI: | 10.1016/j.redox.2014.10.005 |