دورية أكاديمية
Use of MALDI-TOF for identification and surveillance of gram-negative bacteria in captive wild psittacines
| العنوان: | Use of MALDI-TOF for identification and surveillance of gram-negative bacteria in captive wild psittacines |
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| المؤلفون: | Y. M. Davies, L. S. Franco, F. B. Barbosa, C. L. Vanin, V. T. M. Gomes, L. Z. Moreno, M. R. F. Barbosa, M. I. Z. Sato, A. M. Moreno, T. Knöbl |
| المصدر: | Brazilian Journal of Biology, Vol 82 (2021) |
| بيانات النشر: | Instituto Internacional de Ecologia, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Science LCC:Biology (General) LCC:Zoology LCC:Botany |
| مصطلحات موضوعية: | parrots, psittacine birds, MALDI -TOF MS, enterobacteria, public health, Science, Biology (General), QH301-705.5, Zoology, QL1-991, Botany, QK1-989 |
| الوصف: | Abstract Microbiological studies of the sanitary and health status of psittacine birds that will be reintroduced is important in evaluating whether these animals act as carriers of pathogenic agents to other animals and humans. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a faster and more accurate method to identify bacteria than conventional microbiology methods. The aim of this study was to evaluate the health status of psittacines housed in captivity, by assessment of Gram-negative bacteria from fecal microbiota through MALDI- TOF MS identification. The results indicate high frequency of Gram-negative bacteria in feces (96.5%), especially from the Enterobacteriaceae family (88.7%). The most prevalent bacteria were Escherichia coli (39.0%), Proteus vulgaris (12.2%), Klebsiella spp. (12.1%) and Raoultella ornithinolytica (8.7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. and Escherichia hermannii were isolated with lower frequency. . All these agents are potentially pathogenic for parrots and can cause systemic infections in other animals and humans. These findings reinforce that MALDI- TOF MS proved to be a rapid and accurate method of identification of the microorganism and evaluation of the health status of psittacines, providing relevant data to assist decision-making regarding the sanitary protocols in wildlife centers, and possible future reintroduction of wild birds. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-4375 1519-6984 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100123&tlng=en; https://doaj.org/toc/1678-4375 |
| DOI: | 10.1590/1519-6984.233523 |
| URL الوصول: | https://doaj.org/article/a526c0436bde476899a08bf12906c6ca |
| رقم الأكسشن: | edsdoj.526c0436bde476899a08bf12906c6ca |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16784375 15196984 |
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| DOI: | 10.1590/1519-6984.233523 |