دورية أكاديمية
Evidence of episodic positive selection in Corynebacterium diphtheriae complex of species and its implementations in identification of drug and vaccine targets
| العنوان: | Evidence of episodic positive selection in Corynebacterium diphtheriae complex of species and its implementations in identification of drug and vaccine targets |
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| المؤلفون: | Marcus Vinicius Canário Viana, Rodrigo Profeta, Janaína Canário Cerqueira, Alice Rebecca Wattam, Debmalya Barh, Artur Silva, Vasco Azevedo |
| المصدر: | PeerJ, Vol 10, p e12662 (2022) |
| بيانات النشر: | PeerJ Inc., 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Biology (General) |
| مصطلحات موضوعية: | Corynebacterium, Positive selection, Drug target, Vaccine target, Medicine, Biology (General), QH301-705.5 |
| الوصف: | Background Within the pathogenic bacterial species Corynebacterium genus, six species that can produce diphtheria toxin (C. belfantii, C. diphtheriae, C. pseudotuberculosis, C. rouxii, C. silvaticum and C. ulcerans) form a clade referred to as the C. diphtheria complex. These species have been found in humans and other animals, causing diphtheria or other diseases. Here we show the results of a genome scale analysis to identify positive selection in protein-coding genes that may have resulted in the adaptations of these species to their ecological niches and suggest drug and vaccine targets. Methods Forty genomes were sampled to represent species, subspecies or biovars of Corynebacterium. Ten phylogenetic groups were tested for positive selection using the PosiGene pipeline, including species and biovars from the C. diphtheria complex. The detected genes were tested for recombination and had their sequences alignments and homology manually examined. The final genes were investigated for their function and a probable role as vaccine or drug targets. Results Nineteen genes were detected in the species C. diphtheriae (two), C. pseudotuberculosis (10), C. rouxii (one), and C. ulcerans (six). Those were found to be involved in defense, translation, energy production, and transport and in the metabolism of carbohydrates, amino acids, nucleotides, and coenzymes. Fourteen were identified as essential genes, and six as virulence factors. Thirteen from the 19 genes were identified as potential drug targets and four as potential vaccine candidates. These genes could be important in the prevention and treatment of the diseases caused by these bacteria. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2167-8359 |
| Relation: | https://peerj.com/articles/12662.pdf; https://peerj.com/articles/12662/; https://doaj.org/toc/2167-8359 |
| DOI: | 10.7717/peerj.12662 |
| URL الوصول: | https://doaj.org/article/527e9cb082e34b34ab534ec7cc7279bb |
| رقم الأكسشن: | edsdoj.527e9cb082e34b34ab534ec7cc7279bb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21678359 |
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| DOI: | 10.7717/peerj.12662 |