دورية أكاديمية
Cervical Intraepithelial Neoplasia grade 2 biopsy: Do p16INK4a and Ki-67 biomarkers contribute to the decision to treat? A cross-sectional study
| العنوان: | Cervical Intraepithelial Neoplasia grade 2 biopsy: Do p16INK4a and Ki-67 biomarkers contribute to the decision to treat? A cross-sectional study |
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| المؤلفون: | Amanda Leal Ferreira, Nasle Domingues Dibe, Bruna Rodrigues de Paiva, Elyzabeth Avvad Portari, Dione Corrêa de Araújo Dock, Nilma Valéria Caldeira Ferreira, Saint Clair Gomes Junior, Fábio Bastos Russomano, Cecília Vianna de Andrade |
| المصدر: | São Paulo Medical Journal, Vol 142, Iss 1 (2023) |
| بيانات النشر: | Associação Paulista de Medicina, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Uterine cervical dysplasia, Cyclin-dependent kinase inhibitor p16, Ki-67 antigen, Cervical intraepithelial neoplasia, Cervical intraepithelial neoplasia grade 2, CIN2, p16INK4a, Ki-67, Medicine |
| الوصف: | ABSTRACT BACKGROUND: Managing cervical intraepithelial neoplasia grade 2 (CIN2) is challenging, considering the CIN2 regression rate, perinatal risks associated with excisional procedures, and insufficient well-established risk factors to predict progression. OBJECTIVES: To determine the ability of p16INK4a and Ki-67 staining in biopsies diagnosed with CIN2 to identify patients with higher-grade lesions (CIN3 or carcinoma). DESIGN AND SETTING: Cross-sectional study conducted at a referral center for treating uterine cervical lesions. METHODS: In 79 women, we analyzed the correlation of p16INK4a and Ki-67 expression in CIN2 biopsies with the presence of a higher-grade lesions, as determined via histopathology in surgical specimens from treated women or via two colposcopies and two cytological tests during follow-up for untreated women with at least a 6-month interval. The expression of these two biomarkers was verified by at least two independent pathologists and quantified using digital algorithms. RESULTS: Thirteen (16.8%) women with CIN2 biopsy exhibited higher-grade lesions on the surgical excision specimen or during follow-up. p16INK4a expression positively and negatively predicted the presence of higher-grade lesions in 17.19% and 86.67% patients, respectively. Ki-67 expression positively and negatively predicted the presence of higher-grade lesions in 40% and 88.24% patients, respectively. CONCLUSIONS: Negative p16INK4a and Ki67 immunohistochemical staining can assure absence of a higher-grade lesion in more than 85% of patients with CIN2 biopsies and can be used to prevent overtreatment of these patients. Positive IHC staining for p16INK4a and Ki-67 did not predict CIN3 in patients with CIN2 biopsies. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1806-9460 1516-3180 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802024000100211&tlng=en; https://doaj.org/toc/1806-9460 |
| DOI: | 10.1590/1516-3180.2022.0527.r2.280423 |
| URL الوصول: | https://doaj.org/article/52a587f4152a43969b23087fd5f87b3b |
| رقم الأكسشن: | edsdoj.52a587f4152a43969b23087fd5f87b3b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 18069460 15163180 |
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| DOI: | 10.1590/1516-3180.2022.0527.r2.280423 |