دورية أكاديمية
Brucella abortus RB51 lipopolysaccharide influence as an adjuvant on the therapeutic efficacy of HPV16 L1 and HPV16 E7 DNA vaccines
| العنوان: | Brucella abortus RB51 lipopolysaccharide influence as an adjuvant on the therapeutic efficacy of HPV16 L1 and HPV16 E7 DNA vaccines |
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| المؤلفون: | Masoumeh Shirmohammadi, Hoorieh Soleimanjahi, Zahra Kianmehr, Hesam Karimi, Sussan Kabudanian Ardestani |
| المصدر: | Iranian Journal of Basic Medical Sciences, Vol 24, Iss 1, Pp 92-97 (2021) |
| بيانات النشر: | Mashhad University of Medical Sciences, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | adjuvant, brucella abortus rb51, cervical cancer, dna vaccines, human papillomavirus, lipopolysaccharide, Medicine |
| الوصف: | Objective(s): Human papillomavirus (HPV) is a primary contributing agent of cervical cancer. Eradication of HPV-related infections requires therapeutic strategies. We used Brucella abortus RB51 rough lipopolysaccharide (R-LPS) as an adjuvant along with two HPV16 therapeutic DNA vaccines, pcDNA3-E7 and pcDNA3-L1, for improving DNA vaccine efficacy. Materials and Methods: For evaluation of the B. abortus LPS adjuvant efficacy in combination with DNA vaccines to induce cellular immune responses, C57BL/6 mice were immunized with the DNA vaccines, with or without R-LPS adjuvant. IFN-γ and IL-4 cytokines assay was carried out for assessment of cellular and humoral immune responses. Results: Findings indicated that vaccination with pcDNA3-E7 or pcDNA3-L1 alone could induce strong cellular immune responses, but stronger antigen-specific T-cell immune responses were shown by co-administration of HPV16 E7 and HPV16 L1 DNA vaccines along with R-LPS adjuvant. Conclusion: Overall, B. abortus R-LPS through enhancement of T-cell immune responses can be considered an efficient vaccine adjuvant in future studies and trials. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2008-3866 2008-3874 |
| Relation: | https://ijbms.mums.ac.ir/article_17049_c655752d096af5d58706ad4efdf37fba.pdf; https://doaj.org/toc/2008-3866; https://doaj.org/toc/2008-3874 |
| DOI: | 10.22038/ijbms.2020.51043.11608 |
| URL الوصول: | https://doaj.org/article/5308ed87a49c488baf03868b2a249b65 |
| رقم الأكسشن: | edsdoj.5308ed87a49c488baf03868b2a249b65 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20083866 20083874 |
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| DOI: | 10.22038/ijbms.2020.51043.11608 |