دورية أكاديمية
Cloning, Characterization and Functional Analysis of Caspase 8-like Gene in Apoptosis of Crassostrea hongkongensis Response to Hyper-Salinity Stress
| العنوان: | Cloning, Characterization and Functional Analysis of Caspase 8-like Gene in Apoptosis of Crassostrea hongkongensis Response to Hyper-Salinity Stress |
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| المؤلفون: | Jinji Lin, Ziqi Yu, Yang Leng, Jiexiong Zhu, Feifei Yu, Yishan Lu, Jiayu Chen, Wenhao He, Yixin Zhang, Yaoshen Wen |
| المصدر: | Fishes, Vol 9, Iss 5, p 172 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Genetics |
| مصطلحات موضوعية: | Crassostrea hongkongensis, caspase8-like, apoptosis, hyper-salinity stress, Biology (General), QH301-705.5, Genetics, QH426-470 |
| الوصف: | Caspase-8, a member of the caspase family, is an initiating caspase and plays a crucial role in apoptosis. In this study, the full-length cDNA of caspase8-like (CASP8-like) was isolated from Crassostrea hongkongensis (C. hongkongensis) by RACE-PCR. ChCASP8-like contained a 1599-bp open reading frame (ORF) encoding 533 amino acids with two conserved death effector domains (DEDs) and a cysteine aspartase cysteine structural domain (CASc). Amino acid sequence comparison showed that ChCASP8-like shared the highest identity (85.4%) with CASP8-like of C. angulata. The tissue expression profile showed that ChCASP8-like was constitutively expressed in gills, hepatopancreas, mantle, adductor muscle, hemocytes and gonads, and was significantly upregulated in hemocytes, hepatopancreas and gills under hyper-salinity stress. The apoptosis-related genes, including ATR, CHK1, BCL-XL, CASP8-like, CASP9 and CASP3, were significantly activated by hyper-salinity stress, but were remarkably inhibited by ChCASP8-like silencing. The caspase 8 activity was increased by 1.7-fold after hyper-salinity stress, and was inhibited by 9.4% by ChCASP8-like silencing. Moreover, ChCASP8-like silencing clearly alleviated the apoptosis resulting from hyper-salinity stress. These results collectively demonstrated that ChCASP8-like played a crucial role in inducing apoptosis against hyper-salinity stress. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2410-3888 |
| Relation: | https://www.mdpi.com/2410-3888/9/5/172; https://doaj.org/toc/2410-3888 |
| DOI: | 10.3390/fishes9050172 |
| URL الوصول: | https://doaj.org/article/531c11433f50414c8d51ad3254e3ca98 |
| رقم الأكسشن: | edsdoj.531c11433f50414c8d51ad3254e3ca98 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24103888 |
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| DOI: | 10.3390/fishes9050172 |