دورية أكاديمية
High-throughput proteomics of nanogram-scale samples with Zeno SWATH MS
العنوان: | High-throughput proteomics of nanogram-scale samples with Zeno SWATH MS |
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المؤلفون: | Ziyue Wang, Michael Mülleder, Ihor Batruch, Anjali Chelur, Kathrin Textoris-Taube, Torsten Schwecke, Johannes Hartl, Jason Causon, Jose Castro-Perez, Vadim Demichev, Stephen Tate, Markus Ralser |
المصدر: | eLife, Vol 11 (2022) |
بيانات النشر: | eLife Sciences Publications Ltd, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
مصطلحات موضوعية: | proteomics, high-throughput screening, liquid chromatography, data-independent acquisition, Medicine, Science, Biology (General), QH301-705.5 |
الوصف: | The possibility to record proteomes in high throughput and at high quality has opened new avenues for biomedical research, drug discovery, systems biology, and clinical translation. However, high-throughput proteomic experiments often require high sample amounts and can be less sensitive compared to conventional proteomic experiments. Here, we introduce and benchmark Zeno SWATH MS, a data-independent acquisition technique that employs a linear ion trap pulsing (Zeno trap pulsing) to increase the sensitivity in high-throughput proteomic experiments. We demonstrate that when combined with fast micro- or analytical flow-rate chromatography, Zeno SWATH MS increases protein identification with low sample amounts. For instance, using 20 min micro-flow-rate chromatography, Zeno SWATH MS identified more than 5000 proteins consistently, and with a coefficient of variation of 6%, from a 62.5 ng load of human cell line tryptic digest. Using 5 min analytical flow-rate chromatography (800 µl/min), Zeno SWATH MS identified 4907 proteins from a triplicate injection of 2 µg of a human cell lysate, or more than 3000 proteins from a 250 ng tryptic digest. Zeno SWATH MS hence facilitates sensitive high-throughput proteomic experiments with low sample amounts, mitigating the current bottlenecks of high-throughput proteomics. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2050-084X |
Relation: | https://elifesciences.org/articles/83947; https://doaj.org/toc/2050-084X |
DOI: | 10.7554/eLife.83947 |
URL الوصول: | https://doaj.org/article/c5329d8f8bc14be1b785b5780257442e |
رقم الأكسشن: | edsdoj.5329d8f8bc14be1b785b5780257442e |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 2050084X |
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DOI: | 10.7554/eLife.83947 |