دورية أكاديمية
Substrate stiffness governs the initiation of B cell activation by the concerted signaling of PKCβ and focal adhesion kinase
| العنوان: | Substrate stiffness governs the initiation of B cell activation by the concerted signaling of PKCβ and focal adhesion kinase |
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| المؤلفون: | Samina Shaheen, Zhengpeng Wan, Zongyu Li, Alicia Chau, Xinxin Li, Shaosen Zhang, Yang Liu, Junyang Yi, Yingyue Zeng, Jing Wang, Xiangjun Chen, Liling Xu, Wei Chen, Fei Wang, Yun Lu, Wenjie Zheng, Yan Shi, Xiaolin Sun, Zhanguo Li, Chunyang Xiong, Wanli Liu |
| المصدر: | eLife, Vol 6 (2017) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | Mechanosensing, B cell, Substrate Stiffness, PKCβ, Focal adhesion kinase, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | The mechanosensing ability of lymphocytes regulates their activation in response to antigen stimulation, but the underlying mechanism remains unexplored. Here, we report that B cell mechanosensing-governed activation requires BCR signaling molecules. PMA-induced activation of PKCβ can bypass the Btk and PLC-γ2 signaling molecules that are usually required for B cells to discriminate substrate stiffness. Instead, PKCβ-dependent activation of FAK is required, leading to FAK-mediated potentiation of B cell spreading and adhesion responses. FAK inactivation or deficiency impaired B cell discrimination of substrate stiffness. Conversely, adhesion molecules greatly enhanced this capability of B cells. Lastly, B cells derived from rheumatoid arthritis (RA) patients exhibited an altered BCR response to substrate stiffness in comparison with healthy controls. These results provide a molecular explanation of how initiation of B cell activation discriminates substrate stiffness through a PKCβ-mediated FAK activation dependent manner. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/23060; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.23060 |
| URL الوصول: | https://doaj.org/article/53442122ceb94add81f255cfd8082d1e |
| رقم الأكسشن: | edsdoj.53442122ceb94add81f255cfd8082d1e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.23060 |