دورية أكاديمية
Iron Oxide Nanoparticles Carrying 5-Fluorouracil in Combination with Magnetic Hyperthermia Induce Thrombogenic Collagen Fibers, Cellular Stress, and Immune Responses in Heterotopic Human Colon Cancer in Mice
العنوان: | Iron Oxide Nanoparticles Carrying 5-Fluorouracil in Combination with Magnetic Hyperthermia Induce Thrombogenic Collagen Fibers, Cellular Stress, and Immune Responses in Heterotopic Human Colon Cancer in Mice |
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المؤلفون: | Mohammad Dabaghi, Seyed Mohammad Mahdi Rasa, Emilio Cirri, Alessandro Ori, Francesco Neri, Rainer Quaas, Ingrid Hilger |
المصدر: | Pharmaceutics, Vol 13, Iss 10, p 1625 (2021) |
بيانات النشر: | MDPI AG, 2021. |
سنة النشر: | 2021 |
المجموعة: | LCC:Pharmacy and materia medica |
مصطلحات موضوعية: | magnetic hyperthermia, thermal treatment, nanoparticles, colon cancer, DAMPs, thrombogenic collagen fibers, Pharmacy and materia medica, RS1-441 |
الوصف: | In this study we looked for the main protein pathway regulators which were responsible for the therapeutic impact on colon cancers when combining magnetic hyperthermia with the chemotherapeutic agent 5-fluorouracil (5FU). To this end, chitosan-coated magnetic nanoparticles (MNP) functionalized with 5FU were intratumorally injected into subcutaneous human colon cancer xenografts (HT-29) in mice and exposed to an alternating magnetic field. A decreased tumor growth was found particularly for the combined thermo-chemotherapy vs. the corresponding monotherapies. By using computational analysis of the tumor proteome, we found upregulated functional pathway categories termed “cellular stress and injury”, “intracellular second messenger and nuclear receptor signaling”, “immune responses”, and “growth proliferation and development”. We predict TGF-beta, and other mediators, as important upstream regulators. In conclusion, our findings show that the combined thermo-chemotherapy induces thrombogenic collagen fibers which are able to impair tumor nutrient supply. Further on, we associate several responses to the recognition of damage associated molecular patterns (DAMPs) by phagocytic cells, which immigrate into the tumor area. The activation of some pathways associated with cell survival implies the necessity to conduct multiple therapy sessions in connection with a corresponding monitoring, which could possibly be performed on the base of the identified protein regulators. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 13101625 1999-4923 |
Relation: | https://www.mdpi.com/1999-4923/13/10/1625; https://doaj.org/toc/1999-4923 |
DOI: | 10.3390/pharmaceutics13101625 |
URL الوصول: | https://doaj.org/article/536977fbce314dfebcddc733341a83d5 |
رقم الأكسشن: | edsdoj.536977fbce314dfebcddc733341a83d5 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 13101625 19994923 |
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DOI: | 10.3390/pharmaceutics13101625 |