دورية أكاديمية
Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface‐Enhanced Raman Spectroscopy
| العنوان: | Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface‐Enhanced Raman Spectroscopy |
|---|---|
| المؤلفون: | Taek Lee, Mohsen Mohammadniaei, Hui Zhang, Jinho Yoon, Hye Kyu Choi, Sijin Guo, Peixuan Guo, Jeong‐Woo Choi |
| المصدر: | Advanced Science, Vol 7, Iss 3, Pp n/a-n/a (2020) |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Science |
| مصطلحات موضوعية: | biosensors, electrochemical surface‐enhanced Raman spectroscopy (EC‐SERS), microRNAs, monofunctionalization, pRNA 3WJ, Science |
| الوصف: | Abstract Controlling the selective one‐to‐one conjugation of RNA with nanoparticles is vital for future applications of RNA nanotechnology. Here, the monofunctionalization of a gold nanoparticle (AuNP) with a single copy of RNA is developed for ultrasensitive microRNA‐155 quantification using electrochemical surface‐enhanced Raman spectroscopy (EC‐SERS). A single AuNP is conjugated with one copy of the packaging RNA (pRNA) three‐way junction (RNA 3WJ). pRNA 3WJ containing one strand of the 3WJ is connected to a Sephadex G100 aptamer and a biotin group at each arm (SEPapt/3WJ/Bio) which is then immobilized to the Sephadex G100 resin. The resulting complex is connected to streptavidin‐coated AuNP (STV/AuNP). Next, the STV/AuNP–Bio/3WJa is purified and reassembled with another 3WJ to form a single‐labeled 3WJ/AuNP. Later, the monoconjugate is immobilized onto the AuNP‐electrodeposited indium tin oxide coated substrate for detecting microRNA‐155 based on EC‐SERS. Application of an optimum potential of +0.2 V results in extraordinary amplification (≈7 times) of methylene blue (reporter) SERS signal compared to the normal SERS signal. As a result, a highly sensitive detection of 60 × 10−18 m microRNA‐155 in 1 h in serum based on monoconjugated AuNP/RNA is achieved. Thus, the monofunctionalization of RNA onto nanoparticle can provide a new methodology for biosensor construction and diverse RNA nanotechnology development. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2198-3844 20190247 |
| Relation: | https://doaj.org/toc/2198-3844 |
| DOI: | 10.1002/advs.201902477 |
| URL الوصول: | https://doaj.org/article/5381f0d4f7dd48469e705317a683e0b4 |
| رقم الأكسشن: | edsdoj.5381f0d4f7dd48469e705317a683e0b4 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21983844 20190247 |
|---|---|
| DOI: | 10.1002/advs.201902477 |