دورية أكاديمية
Improving Fibrin Hydrogels’ Mechanical Properties, through Addition of Silica or Chitosan-Silica Materials, for Potential Application as Wound Dressings
| العنوان: | Improving Fibrin Hydrogels’ Mechanical Properties, through Addition of Silica or Chitosan-Silica Materials, for Potential Application as Wound Dressings |
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| المؤلفون: | Natalia Y. Becerra, Luz M. Restrepo, Yessika Galeano, Ana C. Tobón, Luis F. Turizo, Monica Mesa |
| المصدر: | International Journal of Biomaterials, Vol 2021 (2021) |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | Biotechnology, TP248.13-248.65 |
| الوصف: | Fibrin is a protein-based hydrogel formed during blood coagulation. It can also be produced in vitro from human blood plasma, and it is capable of resisting high deformations. However, after each deformation process, it loses high amounts of water, which subsequently makes it mechanically unstable and, finally, difficult to manipulate. The objective of this work was to overcome the in vitro fibrin mechanical instability. The strategy consists of adding silica or chitosan-silica materials and comparing how the different materials electrokinetic-surface properties affect the achieved improvement. The siliceous materials electrostatic and steric stabilization mechanisms, together with plasma protein adsorption on their surfaces, were corroborated by DLS and ζ-potential measurements before fibrin gelling. These properties avoid phase separation, favoring homogeneous incorporation of the solid into the forming fibrin network. Young’s modulus of modified fibrin hydrogels was evaluated by AFM to quantitatively measure stiffness. It increased 2.5 times with the addition of 4 mg/mL silica. A similar improvement was achieved with only 0.7 mg/mL chitosan-silica, which highlighted the contribution of hydrophilic chitosan chains to fibrinogen crosslinking. Moreover, these chains avoided the fibroblast growth inhibition onto modified fibrin hydrogels 3D culture observed with silica. In conclusion, 0.7 mg/mL chitosan-silica improved the mechanical stability of fibrin hydrogels with low risks of cytotoxicity. This easy-to-manipulate modified fibrin hydrogel makes it suitable as a wound dressing biomaterial. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1687-8787 1687-8795 |
| Relation: | https://doaj.org/toc/1687-8787; https://doaj.org/toc/1687-8795 |
| DOI: | 10.1155/2021/9933331 |
| URL الوصول: | https://doaj.org/article/538dea7083f84b6690cbb02007c0b09f |
| رقم الأكسشن: | edsdoj.538dea7083f84b6690cbb02007c0b09f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16878787 16878795 |
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| DOI: | 10.1155/2021/9933331 |