دورية أكاديمية
Differential Expression of miR-101 and miR-744 in Nasopharyngeal Carcinoma in Pahang State of Malaysia
| العنوان: | Differential Expression of miR-101 and miR-744 in Nasopharyngeal Carcinoma in Pahang State of Malaysia |
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| المؤلفون: | Azmir AHMAD, Haziq Abdul WAHID, Wan Ishlah LEMAN, Noor Syamila OTHMAN, Azlina Abdul RAHMAN, Kahairi ABDULLAH, Siti Aesah Naznin MUHAMMAD, Mohd. Arifin KADERI |
| المصدر: | Walailak Journal of Science and Technology, Vol 16, Iss 5 (2018) |
| بيانات النشر: | Walailak University, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Technology (General) LCC:Science (General) |
| مصطلحات موضوعية: | Nasopharyngeal carcinoma, miR-101, miR-744, differential expression, diagnostic biomarker, Technology (General), T1-995, Science (General), Q1-390 |
| الوصف: | Previous study found that microRNA-101 (miR-101) and microRNA-744 (miR-744) were deregulated in head and neck cancers and were implicated in nasopharyngeal carcinoma (NPC) carcinogenesis. Thus, this study aimed to determine the expression of miR-101 and miR-744 in NPC and analyse the utility of these microRNAs (miRNAs) as diagnostic biomarkers. Total RNA was extracted from 31 NPC and 7 non-NPC control formalin-fixed paraffin-embedded (FFPE) samples. Complementary DNA (cDNA) was synthesized from the total RNA and proceeded with quantitative real-time polymerase chain reaction. Differential expression of miR-101 and miR-744 were calculated from quantification cycle (Cq) data using 2-ΔΔCq calculation. The performance of these miRNAs were calculated using receiver operating characteristic (ROC) curve analysis. The differential expression for miR-101 and miR-744 were -1.39 (p < 0.05) and 2.48 (p > 0.05), respectively, where the deregulations were consistent with the previous report. The area under curve for miR-101, miR-744 and combination of miR-101 and miR-744 were 0.654 (95 % CI: 0.465 - 0.844), 0.588 (95 % CI: 0.368 - 0.808) and 0.626 (95 % CI: 0.481 - 0.771), respectively. However, re-analysis using balanced sample size between NPC and non-NPC control group showed the value decreased to 0.653 (95 % CI: 0.347 - 0.959) for miR-101 but increased to 0.827 (95 % CI: 0.601 - 1.000) for miR-744 and 0.758 (95 % CI: 0.576 - 0.939) for the combination of miR-101 and miR-744, indicating the importance of having a balanced sample size. We have successfully determined the expression of miR-101 and miR-744 in NPC samples. We also demonstrated statistically the utility of these miRNAs as diagnostic biomarkers. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1686-3933 2228-835X |
| Relation: | http://wjst.wu.ac.th/index.php/wjst/article/view/6285; https://doaj.org/toc/1686-3933; https://doaj.org/toc/2228-835X |
| DOI: | 10.14456/vol16iss5ppCorrected |
| URL الوصول: | https://doaj.org/article/53c0fc53779d477592627ab588913560 |
| رقم الأكسشن: | edsdoj.53c0fc53779d477592627ab588913560 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16863933 2228835X |
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| DOI: | 10.14456/vol16iss5ppCorrected |