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The Effects of CoCl2 on HIF-1α Protein under Experimental Conditions of Autoprogressive Hypoxia Using Mouse Models

التفاصيل البيبلوغرافية
العنوان: The Effects of CoCl2 on HIF-1α Protein under Experimental Conditions of Autoprogressive Hypoxia Using Mouse Models
المؤلفون: Yan-Bo Zhang, Xiulian Wang, Edward A. Meister, Ke-Rui Gong, Shao-Chun Yan, Guo-Wei Lu, Xun-Ming Ji, Guo Shao
المصدر: International Journal of Molecular Sciences, Vol 15, Iss 6, Pp 10999-11012 (2014)
بيانات النشر: MDPI AG, 2014.
سنة النشر: 2014
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: hypoxia preconditioning, hypoxia-inducible factor-1, hippocampus, CoCl2, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: It is well known that cobalt chloride (CoCl2) can enhance the stability of hypoxia-inducible factor (HIF)-1α. The aim of this study is to detect the effect of CoCl2 on the hypoxia tolerance of mice which were repeatedly exposed to autoprogressive hypoxia. Balb/c mice were randomly divided into groups of chemical pretreatment and normal saline (NS), respectively injected with CoCl2 and NS 3 h before exposure to hypoxia for 0 run (H0), 1 run (H1), and 4 runs (H4). Western Blot, electrophoretic mobility shift assay (EMSA), extracellular recordings population spikes in area cornus ammonis I (CA 1) of mouse hippocampal slices and real-time were used in this study. Our results demonstrated that the tolerance of mice to hypoxia, the changes of HIF-1α protein level and HIF-1 DNA binding activity in mice hippocampus, the mRNA level of erythropoietin (EPO) and vascular endothelial growth factor (VEGF), and the disappearance time of population spikes of hippocampal slices were substantially different between the control group and the CoCl2 group. Over-induction of HIF-1α by pretreatment with CoCl2 before hypoxia did not increase the hypoxia tolerance.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: http://www.mdpi.com/1422-0067/15/6/10999; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms150610999
URL الوصول: https://doaj.org/article/e53e2bc485574305940b8221a5e81ca9
رقم الأكسشن: edsdoj.53e2bc485574305940b8221a5e81ca9
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms150610999