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Exogenous aralar/slc25a12 can replace citrin/slc25a13 as malate aspartate shuttle component in liver

التفاصيل البيبلوغرافية
العنوان: Exogenous aralar/slc25a12 can replace citrin/slc25a13 as malate aspartate shuttle component in liver
المؤلفون: Luis González-Moreno, Andrea Santamaría-Cano, Alberto Paradela, María Luz Martínez-Chantar, Miguel Á. Martín, Mercedes Pérez-Carreras, Alberto García-Picazo, Jesús Vázquez, Enrique Calvo, Gloria González-Aseguinolaza, Takeyori Saheki, Araceli del Arco, Jorgina Satrústegui, Laura Contreras
المصدر: Molecular Genetics and Metabolism Reports, Vol 35, Iss , Pp 100967- (2023)
بيانات النشر: Elsevier, 2023.
سنة النشر: 2023
المجموعة: LCC:Medicine (General)
LCC:Biology (General)
مصطلحات موضوعية: Mitochondria, Citrin deficiency, Aspartate-glutamate carrier, Malate-aspartate shuttle, Hepatocyte, Medicine (General), R5-920, Biology (General), QH301-705.5
الوصف: The deficiency of CITRIN, the liver mitochondrial aspartate–glutamate carrier (AGC), is the cause of four human clinical phenotypes, neonatal intrahepatic cholestasis caused by CITRIN deficiency (NICCD), silent period, failure to thrive and dyslipidemia caused by CITRIN deficiency (FTTDCD), and citrullinemia type II (CTLN2). Clinical symptoms can be traced back to disruption of the malate-aspartate shuttle due to the lack of citrin. A potential therapy for this condition is the expression of aralar, the AGC present in brain, to replace citrin. To explore this possibility we have first verified that the NADH/NAD+ ratio increases in hepatocytes from citrin(−/−) mice, and then found that exogenous aralar expression reversed the increase in NADH/NAD+ observed in these cells. Liver mitochondria from citrin (−/−) mice expressing liver specific transgenic aralar had a small (~ 4–6 nmoles x mg prot−1 x min−1) but consistent increase in malate aspartate shuttle (MAS) activity over that of citrin(−/−) mice. These results support the functional replacement between AGCs in the liver. To explore the significance of AGC replacement in human therapy we studied the relative levels of citrin and aralar in mouse and human liver through absolute quantification proteomics. We report that mouse liver has relatively high aralar levels (citrin/aralar molar ratio of 7.8), whereas human liver is virtually devoid of aralar (CITRIN/ARALAR ratio of 397). This large difference in endogenous aralar levels partly explains the high residual MAS activity in liver of citrin(−/−) mice and why they fail to recapitulate the human disease, but supports the benefit of increasing aralar expression to improve the redox balance capacity of human liver, as an effective therapy for CITRIN deficiency.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2214-4269
Relation: http://www.sciencedirect.com/science/article/pii/S2214426923000137; https://doaj.org/toc/2214-4269
DOI: 10.1016/j.ymgmr.2023.100967
URL الوصول: https://doaj.org/article/5453daac2dbc4e41bb3cd39e84ebf7cc
رقم الأكسشن: edsdoj.5453daac2dbc4e41bb3cd39e84ebf7cc
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:22144269
DOI:10.1016/j.ymgmr.2023.100967