دورية أكاديمية
A tug of war between filament treadmilling and myosin induced contractility generates actin rings
| العنوان: | A tug of war between filament treadmilling and myosin induced contractility generates actin rings |
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| المؤلفون: | Qin Ni, Kaustubh Wagh, Aashli Pathni, Haoran Ni, Vishavdeep Vashisht, Arpita Upadhyaya, Garegin A Papoian |
| المصدر: | eLife, Vol 11 (2022) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | cytoskeleton, actomyosin ring, molecular simulation, T cells, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | In most eukaryotic cells, actin filaments assemble into a shell-like actin cortex under the plasma membrane, controlling cellular morphology, mechanics, and signaling. The actin cortex is highly polymorphic, adopting diverse forms such as the ring-like structures found in podosomes, axonal rings, and immune synapses. The biophysical principles that underlie the formation of actin rings and cortices remain unknown. Using a molecular simulation platform called MEDYAN, we discovered that varying the filament treadmilling rate and myosin concentration induces a finite size phase transition in actomyosin network structures. We found that actomyosin networks condense into clusters at low treadmilling rates or high myosin concentrations but form ring-like or cortex-like structures at high treadmilling rates and low myosin concentrations. This mechanism is supported by our corroborating experiments on live T cells, which exhibit ring-like actin networks upon activation by stimulatory antibody. Upon disruption of filament treadmilling or enhancement of myosin activity, the pre-existing actin rings are disrupted into actin clusters or collapse towards the network center respectively. Our analyses suggest that the ring-like actin structure is a preferred state of low mechanical energy, which is, importantly, only reachable at sufficiently high treadmilling rates. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/82658; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.82658 |
| URL الوصول: | https://doaj.org/article/545608be2de84536ba0c939a1640cd5f |
| رقم الأكسشن: | edsdoj.545608be2de84536ba0c939a1640cd5f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.82658 |