دورية أكاديمية
Cypress tree (Chamaecyparis obtusa) Bark extract inhibits melanogenesis through repressing CREB and MITF signalling pathways in α-MSH-stimulated B16F10 cells
| العنوان: | Cypress tree (Chamaecyparis obtusa) Bark extract inhibits melanogenesis through repressing CREB and MITF signalling pathways in α-MSH-stimulated B16F10 cells |
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| المؤلفون: | Al Borhan Bayazid, Young Ah Jang, Soo Ah Jeong, Beong Ou Lim |
| المصدر: | Food and Agricultural Immunology, Vol 33, Iss 1, Pp 498-510 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Agriculture (General) LCC:Immunologic diseases. Allergy |
| مصطلحات موضوعية: | Cypress tree bark, B16F10 cells, melanogenesis, CREB/MITF: tyrosinase, Agriculture (General), S1-972, Immunologic diseases. Allergy, RC581-607 |
| الوصف: | Cypress tree (Chamaecyparis obtusa) bark is well-known for its bio-functional activities and high content of polyphenol and flavonoids. It has previously exhibited antioxidant, anti-pathogenic, and anti-inflammatory activities. Our study aimed to investigate the anti-melanogenic effect of Cypress Tree Bark extract (CBE). We evaluated cellular tyrosinase activity and melanin content in alpha-melanocyte-stimulating hormone (α-MSH) induced B16F10 murine melanoma cells. We analyzed microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP1 and TRP2), and cAMP response element-binding protein (CREB) activation via phosphorylation of AKT and ERK using western blot analysis. Tyrosinase, MITF, TRP1, and TRP2 mRNA expression were examined via real-time polymerase chain reaction. CBE restored melanin content and tyrosinase activity remarkably in α-MSH stimulated melanoma cells. It exhibited an anti-melanogenic effect through suppressing MITF, TRP1, TRP2, tyrosinase mRNA and protein expression in α-MSH-induced B16F10 cells. Furthermore, CBE has significantly inhibited CREB activation by suppressing AKT and extracellular signal-regulated kinase phosphorylation. Our data strongly suggest that CBE has potential effects against melanogenesis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 09540105 1465-3443 0954-0105 |
| Relation: | https://doaj.org/toc/0954-0105; https://doaj.org/toc/1465-3443 |
| DOI: | 10.1080/09540105.2022.2095986 |
| URL الوصول: | https://doaj.org/article/e54f2e0835564606993abdbeda59f24e |
| رقم الأكسشن: | edsdoj.54f2e0835564606993abdbeda59f24e |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 09540105 14653443 |
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| DOI: | 10.1080/09540105.2022.2095986 |