دورية أكاديمية
In vivo generated human CAR T cells eradicate tumor cells
العنوان: | In vivo generated human CAR T cells eradicate tumor cells |
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المؤلفون: | Shiwani Agarwal, Tatjana Weidner, Frederic B. Thalheimer, Christian J. Buchholz |
المصدر: | OncoImmunology, Vol 8, Iss 12 (2019) |
بيانات النشر: | Taylor & Francis Group, 2019. |
سنة النشر: | 2019 |
المجموعة: | LCC:Immunologic diseases. Allergy LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
مصطلحات موضوعية: | cell therapy, car t cells, in vivo gene delivery, receptor targeting, t-cell targeting, Immunologic diseases. Allergy, RC581-607, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
الوصف: | Chimeric antigen receptor (CAR) T cells are in prime focus of current research in cancer immunotherapy. Facilitating CAR T cell generation is among the top goals. We have recently demonstrated direct in vivo generation of human CD19-CAR T cells by targeting CD8+ cells using lentiviral vectors (LVs). The anti-tumor potency of in vivo generated CAR T cells was assessed in human PBMC-transplanted NSG mice carrying i.v. injected CD19+ Nalm-6 tumor cells. A single injection of CD8-targeted LV delivering CD19-CAR was sufficient to completely eliminate the tumor cells from bone marrow and spleen, whereas control animals contained high levels of CD19+ cells. Tumor elimination was due to in vivo generated CAR+ cells. Notably, these were not only composed of T lymphocytes but also included CAR+ natural killer cells (NK and NKT). This is the first demonstration of tumor elimination by in vivo generated human CAR T cells. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2162-402X 2162402X |
Relation: | https://doaj.org/toc/2162-402X |
DOI: | 10.1080/2162402X.2019.1671761 |
URL الوصول: | https://doaj.org/article/a54ffa6b658d4c4997a6875352cc2e76 |
رقم الأكسشن: | edsdoj.54ffa6b658d4c4997a6875352cc2e76 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 2162402X |
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DOI: | 10.1080/2162402X.2019.1671761 |