دورية أكاديمية
Effect of alteplase on platelet function and receptor expression
| العنوان: | Effect of alteplase on platelet function and receptor expression |
|---|---|
| المؤلفون: | Jun Lu, Peng Hu, Guangyu Wei, Qi Luo, Jianlin Qiao, Deqin Geng |
| المصدر: | Journal of International Medical Research, Vol 47 (2019) |
| بيانات النشر: | SAGE Publishing, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine (General) |
| مصطلحات موضوعية: | Medicine (General), R5-920 |
| الوصف: | Objective To investigate the role of alteplase, a widely-used thrombolytic drug, in platelet function. Methods Human platelets were incubated with different concentrations of alteplase followed by analysis of platelet aggregation in response to adenosine diphosphate (ADP), collagen, ristocetin, arachidonic acid or epinephrine using light transmittance aggregometry. Platelet activation and surface levels of platelet receptors GPIbα, GPVI and αIIbβ3 were analysed using flow cytometry. The effect of alteplase on clot retraction was also examined. Results This study demonstrated that alteplase significantly inhibited platelet aggregation in response to ADP, collagen and epinephrine in a dose-dependent manner, but it did not affect ristocetin- or arachidonic acid-induced platelet aggregation. Alteplase did not affect platelet activation as demonstrated by no differences in P-selectin levels and PAC-1 binding being observed in collagen-stimulated platelets after alteplase treatment compared with vehicle. There were no changes in the surface levels of the platelet receptors GPIbα, GPVI and αIIbβ3 in alteplase-treated platelets. Alteplase treatment reduced thrombin-mediated clot retraction. Conclusions Alteplase inhibits platelet aggregation and clot retraction without affecting platelet activation and surface receptor levels. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0300-0605 1473-2300 03000605 |
| Relation: | https://doaj.org/toc/0300-0605; https://doaj.org/toc/1473-2300 |
| DOI: | 10.1177/0300060519829991 |
| URL الوصول: | https://doaj.org/article/5559caf555cc4c3a8fbd5eb8ab81a6ed |
| رقم الأكسشن: | edsdoj.5559caf555cc4c3a8fbd5eb8ab81a6ed |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 03000605 14732300 |
|---|---|
| DOI: | 10.1177/0300060519829991 |