دورية أكاديمية
Understanding optical reflectance contrast for real‐time characterization of epithelial precursor lesions
| العنوان: | Understanding optical reflectance contrast for real‐time characterization of epithelial precursor lesions |
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| المؤلفون: | Si Chen, Xin Ge, Xinyu Liu, Qianshan Ding, Nanshuo Wang, Xianghong Wang, Shufen Chen, Haitao Liang, Yunchao Deng, Qiaozhou Xiong, Guangming Ni, En Bo, Chenjie Xu, Honggang Yu, Linbo Liu |
| المصدر: | Bioengineering & Translational Medicine, Vol 4, Iss 3, Pp n/a-n/a (2019) |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Chemical engineering LCC:Biotechnology LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | epithelial cancer, keratin filament, mucin granule, optical biopsy, optical coherence tomography, optical reflectance contrast, Chemical engineering, TP155-156, Biotechnology, TP248.13-248.65, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Abstract Detecting early‐stage epithelial cancers and their precursor lesions are challenging as lesions could be subtle and focally or heterogeneously distributed over large mucosal areas. Optical coherence tomography (OCT) that enables wide‐field imaging of subsurface microstructures in vivo is a promising screening tool for epithelial diseases. However, its diagnostic capability has not been fully appreciated since the optical reflectance contrast is poorly understood. We investigated the back‐scattered intensities from clustered or packed nanometer scale intracellular scatterers using finite‐difference time‐domain method and 1‐μm resolution form of OCT, and uncovered that there existed correlations between the reflectance contrasts and the ultrastructural clustering or packing states of these scatterers, which allows us to interpret the physiological state of the cells. Specifically, both polarized goblet cells and foveolar cells exhibited asymmetric reflectance contrast, but they could be differentiated by the optical intensity of the mucin cup due to the different ultrastructural make‐ups of the mucin granules; keratinocytes could demonstrate varied cytoplasmic intensity and their cytoplasmic contrast was closely correlated with the packing state of keratin filaments. Further preliminary study demonstrated that these new understandings of OCT image contrast enables the characterization of precancerous lesions, which could complement the current morphology‐based criteria in realizing “virtual histology” and would have a profound impact for the screening and surveillance of epithelial cancers. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2380-6761 |
| Relation: | https://doaj.org/toc/2380-6761 |
| DOI: | 10.1002/btm2.10137 |
| URL الوصول: | https://doaj.org/article/560347bb514148ecbf62be084ba824f4 |
| رقم الأكسشن: | edsdoj.560347bb514148ecbf62be084ba824f4 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23806761 |
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| DOI: | 10.1002/btm2.10137 |