دورية أكاديمية
Molecular identification of a root apical cell-specific and stress-responsive enhancer from an Arabidopsis enhancer trap line
| العنوان: | Molecular identification of a root apical cell-specific and stress-responsive enhancer from an Arabidopsis enhancer trap line |
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| المؤلفون: | Lei Zhang, Li-Na Qin, Zi-Rui Zeng, Chang-Zheng Wu, Yuan-Yong Gong, Lai-Hua Liu, Feng-Qiu Cao |
| المصدر: | Plant Methods, Vol 15, Iss 1, Pp 1-11 (2019) |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Plant culture LCC:Biology (General) |
| مصطلحات موضوعية: | Enhancer trap line, Enhancer, Root apex specific expression, GFP and GUS, Abiotic stimuli, Plant culture, SB1-1110, Biology (General), QH301-705.5 |
| الوصف: | Abstract Background Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly at a molecular and cellular level, an Arabidopsis T-DNA insertional enhancer trap line J3411 expressing reporters (GFP) only in the root tip was adopted in this study to isolate a DNA fragment. Results Using nested PCR, DNA sequencing and sequence homology search, the T-DNA insertion site(s) and its flanking genes were characterised in J3411 line. Subsequently, a 2000 bp plant DNA-fragment (Ertip1) upstream of the insert position of the coding T-DNA was in silico analysed, revealing certain putative promoter/enhancer cis-regulatory elements. Cloning and transformation of this DNA fragment and its truncated segments tagged with or without 35S minimal promoter (35Smini), all of which were fused with a GFP or GUS reporter, allowed to detect GFP and GUS expression mediated only by Ertip1 + 35mini (PErtip1+35Smini) specifically in the Arabidopsis root tip region. The PErtip1+35Smini activity was further tested to be strong and stable under many different growth conditions but suppressed by cold, salt, alkaline pH and higher ammonium and phosphorus. Conclusion This work describes a promising strategy to isolate a tissue-/cell-specific enhancer sequence from the enhancer trap lines, which are publically available. The reported synthetic promoter i.e. PErtip1+35Smini may provide a valuable and potent molecular-tool for comprehensive investigation of a gene function related to root growth and development as well as molecular engineering of root-architectural formation aiming to improve plant growth. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1746-4811 |
| Relation: | http://link.springer.com/article/10.1186/s13007-019-0393-0; https://doaj.org/toc/1746-4811 |
| DOI: | 10.1186/s13007-019-0393-0 |
| URL الوصول: | https://doaj.org/article/c564fd4afe294856bf269c5c9d9a59f6 |
| رقم الأكسشن: | edsdoj.564fd4afe294856bf269c5c9d9a59f6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17464811 |
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| DOI: | 10.1186/s13007-019-0393-0 |