دورية أكاديمية
Ultrasound-triggered release of miR-199a-3p from liposome nanobubbles for enhanced hepatocellular carcinoma treatment
| العنوان: | Ultrasound-triggered release of miR-199a-3p from liposome nanobubbles for enhanced hepatocellular carcinoma treatment |
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| المؤلفون: | Xinmin Guo, Jianru Lin, Liwen Pan, Kun He, Zhihui Huang, Jialin Chen, Cuiyan Lin, Baohui Zeng, Sijia Luo, Mengdie Wang |
| المصدر: | Artificial Cells, Nanomedicine, and Biotechnology, Vol 51, Iss 1, Pp 560-571 (2023) |
| بيانات النشر: | Taylor & Francis Group, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Medical technology |
| مصطلحات موضوعية: | Hepatocellular carcinoma, gene therapy, miR-199a-3p, ultrasound-targeted nanobubble destruction, liposome nanobubbles, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| الوصف: | AbstractThis study was aimed to develop an efficient tumour-targeted liposome nanobubbles (LNBs) system using ultrasound-targeted nanobubble destruction for enhanced release and transfection of miRNA-199a-3p in hepatocellular carcinoma (HCC) therapy. The prepared LNBs comprised a polyethylene glycol-modified liposome shell and a perfluoropentane (PFP) core. MiRNA-199a-3p was attached to the nanocomposite surface via electrostatic adsorption, while RGD peptide functionalized the LNBs surface for enhanced HCC cell targeting, namely PFP@miR-RGD-LNBs. The LNBs were spherical with a narrow size distribution. The gene-loaded LNBs effectively condensed miR-199a-3p and protected it from enzymatic degradation. Low-intensity focused ultrasound (LIFU) promoted a fast release of miR-199a-3p from the prepared LNBs, thereby enhancing therapeutic effects. The combined application of PFP@miR-RGD-LNBs and LIFU exhibited a more potent inhibitory effect on HepG2 cells than the other groups, potentially due to LIFU promoting rapid and efficient gene release at the target site and increasing cell membrane permeability. Quantitative reverse transcription-polymerase chain reaction analysis revealed significantly increased mRNA expression levels of key apoptosis markers (Bad, Bax, Caspase-9 and Caspase-3) in the PFP@miR-RGD-LNBs + LIFU group compared to other groups. These findings suggest that the prepared LNBs are highly likely to be promising candidates for further exploration of HCC gene delivery and therapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 21691401 2169-141X 2169-1401 |
| Relation: | https://doaj.org/toc/2169-1401; https://doaj.org/toc/2169-141X |
| DOI: | 10.1080/21691401.2023.2268137 |
| URL الوصول: | https://doaj.org/article/565bc5b5a6054416add4ed3b37816840 |
| رقم الأكسشن: | edsdoj.565bc5b5a6054416add4ed3b37816840 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 21691401 2169141X |
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| DOI: | 10.1080/21691401.2023.2268137 |