دورية أكاديمية
Integrating 5-hydroxymethylcytosine into the epigenomic landscape of human embryonic stem cells.
| العنوان: | Integrating 5-hydroxymethylcytosine into the epigenomic landscape of human embryonic stem cells. |
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| المؤلفون: | Keith E Szulwach, Xuekun Li, Yujing Li, Chun-Xiao Song, Ji Woong Han, SangSung Kim, Sandeep Namburi, Karen Hermetz, Julie J Kim, M Katharine Rudd, Young-Sup Yoon, Bing Ren, Chuan He, Peng Jin |
| المصدر: | PLoS Genetics, Vol 7, Iss 6, p e1002154 (2011) |
| بيانات النشر: | Public Library of Science (PLoS), 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Genetics |
| مصطلحات موضوعية: | Genetics, QH426-470 |
| الوصف: | Covalent modification of DNA distinguishes cellular identities and is crucial for regulating the pluripotency and differentiation of embryonic stem (ES) cells. The recent demonstration that 5-methylcytosine (5-mC) may be further modified to 5-hydroxymethylcytosine (5-hmC) in ES cells has revealed a novel regulatory paradigm to modulate the epigenetic landscape of pluripotency. To understand the role of 5-hmC in the epigenomic landscape of pluripotent cells, here we profile the genome-wide 5-hmC distribution and correlate it with the genomic profiles of 11 diverse histone modifications and six transcription factors in human ES cells. By integrating genomic 5-hmC signals with maps of histone enrichment, we link particular pluripotency-associated chromatin contexts with 5-hmC. Intriguingly, through additional correlations with defined chromatin signatures at promoter and enhancer subtypes, we show distinct enrichment of 5-hmC at enhancers marked with H3K4me1 and H3K27ac. These results suggest potential role(s) for 5-hmC in the regulation of specific promoters and enhancers. In addition, our results provide a detailed epigenomic map of 5-hmC from which to pursue future functional studies on the diverse regulatory roles associated with 5-hmC. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1553-7390 1553-7404 |
| Relation: | http://europepmc.org/articles/PMC3121778?pdf=render; https://doaj.org/toc/1553-7390; https://doaj.org/toc/1553-7404 |
| DOI: | 10.1371/journal.pgen.1002154 |
| URL الوصول: | https://doaj.org/article/d56adc1fb5e94987bfa629575df65a1e |
| رقم الأكسشن: | edsdoj.56adc1fb5e94987bfa629575df65a1e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15537390 15537404 |
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| DOI: | 10.1371/journal.pgen.1002154 |