دورية أكاديمية
Real-Time PCR to Phenotype Resistance to the Citrus Nematode Tylenchulus semipenetrans Cobb.
| العنوان: | Real-Time PCR to Phenotype Resistance to the Citrus Nematode Tylenchulus semipenetrans Cobb. |
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| المؤلفون: | Marta Ruiz, Annie Du Vo, J. Ole Becker, Mikeal L. Roose |
| المصدر: | Plants, Vol 12, Iss 13, p 2543 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Botany |
| مصطلحات موضوعية: | plant parasitic, rootstock, breeding, Poncirus, trifoliate, extraction, Botany, QK1-989 |
| الوصف: | As pest management options, such as nematicides, become more restrictive, developing rootstocks resistant to the citrus nematode is fundamental for citrus production. This study provides an updated methodology to screen for citrus nematode resistance in rootstock-breeding programs. We developed a novel method to extract female citrus nematodes from roots that is suitable for molecular work and a real-time-PCR-based nematode quantification method for Tylenchulus semipenetrans. These procedures allow scaling up screening to high-throughput workflows, increasing the chances of finding rootstock candidates that combine all the desired traits. Our results contribute to the growing literature supporting quantification of nematodes with molecular methods. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 12132543 2223-7747 |
| Relation: | https://www.mdpi.com/2223-7747/12/13/2543; https://doaj.org/toc/2223-7747 |
| DOI: | 10.3390/plants12132543 |
| URL الوصول: | https://doaj.org/article/56e36169dad645c39692849ac66bf291 |
| رقم الأكسشن: | edsdoj.56e36169dad645c39692849ac66bf291 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12132543 22237747 |
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| DOI: | 10.3390/plants12132543 |