دورية أكاديمية
Searching for Biological Function of the Mysterious PA2504 Protein from Pseudomonas aeruginosa
| العنوان: | Searching for Biological Function of the Mysterious PA2504 Protein from Pseudomonas aeruginosa |
|---|---|
| المؤلفون: | Joanna Drabinska, Kamil Steczkiewicz, Martyna Kujawa, Elżbieta Kraszewska |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 18, p 9833 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | PA2504, TUDOR domain, Pseudomonas aeruginosa, sulphur metabolism, crosslink in vivo, RppH, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | For nearly half of the proteome of an important pathogen, Pseudomonas aeruginosa, the function has not yet been recognised. Here, we characterise one such mysterious protein PA2504, originally isolated by us as a sole partner of the RppH RNA hydrolase involved in transcription regulation of multiple genes. This study aims at elucidating details of PA2504 function and discussing its implications for bacterial biology. We show that PA2504 forms homodimers and is evenly distributed in the cytoplasm of bacterial cells. Molecular modelling identified the presence of a Tudor-like domain in PA2504. Transcriptomic analysis of a ΔPA2504 mutant showed that 42 transcripts, mainly coding for proteins involved in sulphur metabolism, were affected by the lack of PA2504. In vivo crosslinking of cellular proteins in the exponential and stationary phase of growth revealed several polypeptides that bound to PA2504 exclusively in the stationary phase. Mass spectrometry analysis identified them as the 30S ribosomal protein S4, the translation elongation factor TufA, and the global response regulator GacA. These results indicate that PA2504 may function as a tether for several important cellular factors. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/22/18/9833; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms22189833 |
| URL الوصول: | https://doaj.org/article/e5769ba43c124636a7e65e3d521aaba9 |
| رقم الأكسشن: | edsdoj.5769ba43c124636a7e65e3d521aaba9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
|---|---|
| DOI: | 10.3390/ijms22189833 |