دورية أكاديمية
Intestinal Epithelial Stem Cells: Distinct Behavior After Surgical Injury and Teduglutide Administration
| العنوان: | Intestinal Epithelial Stem Cells: Distinct Behavior After Surgical Injury and Teduglutide Administration |
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| المؤلفون: | Beatriz P. Costa, Ana C. Gonçalves, Ana M. Abrantes, Raquel Alves, Paulo Matafome, Raquel Seiça, Ana B. Sarmento-Ribeiro, M. Filomena Botelho, Francisco Castro-Sousa |
| المصدر: | Journal of Investigative Surgery, Vol 31, Iss 3, Pp 243-252 (2018) |
| بيانات النشر: | Taylor & Francis Group, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Surgery |
| مصطلحات موضوعية: | intestinal epithelial stem cells, teduglutide, intestinal resection, lgr5, bmi1, flow cytometry, Surgery, RD1-811 |
| الوصف: | Background: Previous studies suggest that intestinal epithelial stem cells (IESC), critical drivers of homeostasis and regeneration, include two subpopulations: crypt-based columnar and “position +4” stem cells, identified by Lgr5 and Bmi1 biomarkers, respectively. Teduglutide is an enterotrophic counterpart of glucagon-like peptide 2. This study aimed to investigate the response of putative IESC to surgical injury and teduglutide administration on an animal model of intestinal resection and anastomosis. Methods: Wistar rats (n = 59) were distributed into four groups: “Ileal Resection” versus “Laparotomy”, subsequently subdivided into “Postoperative Teduglutide Administration” versus “No Treatment”; and sacrificed at third or seventh days, with ileal sample harvesting. Flow cytometry was used to analyze epithelial stem cells with monoclonal antibodies against Lgr5, Bmi1 and also CD44, CD24, CD166, and Grp78 surface markers. Results: Surgical trauma induced an increase of epithelial stem cells population at third day (9.0 ± 0.3 versus 5.7 ± 0.3%, p = 0.0001), which was more intense and involved all subpopulations after ileal resection. At seventh day, teduglutide was significantly associated with higher proportion of Lgr5+/Bmi1− cells (5.8 ± 0.1 versus 2.9 ± 0.3%, p = 0.005) and, on the contrary, lower percentage of Lgr5−/Bmi1+ cells (0.03 ± 0.01 versus 1.9 ± 0.1%, p = 0.049) after ileal resection; and higher proportion of Lgr5+/Bmi1+ cells (1.7 ± 0.1 versus 1.1 ± 0.2%, p = 0.028) after isolated laparotomy. After surgery, Lgr5+/Bmi1− and Lgr5−/Bmi1+ subpopulations demonstrated an inverse correlation and both correlated negatively with Grp78 labeling index. Lgr5−/Bmi1+ and CD44+/CD24low/CD166+/Grp78+ cells proportions exhibited a high grade positive correlation. Conclusion: Those observations support the existence of two epithelial stem cells subpopulations with distinct behavior after surgical injury and teduglutide treatment. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0894-1939 1521-0553 08941939 |
| Relation: | https://doaj.org/toc/0894-1939; https://doaj.org/toc/1521-0553 |
| DOI: | 10.1080/08941939.2017.1294217 |
| URL الوصول: | https://doaj.org/article/a5803e144b9d4664bbe2ca950a1f57d9 |
| رقم الأكسشن: | edsdoj.5803e144b9d4664bbe2ca950a1f57d9 |
| قاعدة البيانات: | Directory of Open Access Journals |
PDF full text from Publisher | تدمد: | 08941939 15210553 |
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| DOI: | 10.1080/08941939.2017.1294217 |