دورية أكاديمية
Effect of Aqueous Extract of Maca Addition to an Extender for Chilled Canine Semen
| العنوان: | Effect of Aqueous Extract of Maca Addition to an Extender for Chilled Canine Semen |
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| المؤلفون: | Chiara Del Prete, Alfonso Calabria, Valentina Longobardi, Veronica Palumbo, Barbara Merlo, Eleonora Iacono, Simona Tafuri, Domenico Carotenuto, Francesca Ciani, Sara Damiano, Roberto Ciarcia, Natascia Cocchia |
| المصدر: | Animals, Vol 12, Iss 13, p 1638 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Veterinary medicine LCC:Zoology |
| مصطلحات موضوعية: | canine spermatozoa, antioxidant, Lepidium meyenii, semen cooling, sperm hyperactivation, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| الوصف: | Antioxidant supplementation has been proposed as a new strategy to improve the long-term preservation of semen. The aim of this study was to evaluate the effect of Maca supplementation of semen extender on quality-related canine semen parameters during cooling. Ejaculates from nine dogs were cooled for 7 days in the absence (control group) or in the presence of 10, 20 and 50 μL/mL of an aqueous extract of Maca. Sperm were evaluated for sperm viability, motility, DNA fragmentation and lipid peroxidation after 3 h, 24 h, 4 days and 7 days of storage. The addition of 10 μL/mL of Maca preserved sperm DNA and plasma membrane integrity at 3 h and increased sperm curvilinear velocity after 24 h. Treatment with 20 and 50 μL/mL of Maca increased the percentage of hyperactivated sperm after 3 h. Moreover, semen treated with 20 μL/mL of Maca decreased lipid peroxidation at 24 h. A significant reduction of sperm DNA and plasma membrane integrity as well as of kinetics parameters between 3 and 24 h of refrigerated storage with the higher concentration tested was observed. Although Maca was not able to protect canine semen with extended refrigeration storage time, it increased hyperactivation and preserved DNA integrity in short-term storage. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2076-2615 |
| Relation: | https://www.mdpi.com/2076-2615/12/13/1638; https://doaj.org/toc/2076-2615 |
| DOI: | 10.3390/ani12131638 |
| URL الوصول: | https://doaj.org/article/585bfde1c48c4e14ac401627341a62cb |
| رقم الأكسشن: | edsdoj.585bfde1c48c4e14ac401627341a62cb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20762615 |
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| DOI: | 10.3390/ani12131638 |