دورية أكاديمية
Type VI secretion system contributes to Enterohemorrhagic Escherichia coli virulence by secreting catalase against host reactive oxygen species (ROS).
العنوان: | Type VI secretion system contributes to Enterohemorrhagic Escherichia coli virulence by secreting catalase against host reactive oxygen species (ROS). |
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المؤلفون: | Baoshan Wan, Qiufen Zhang, Jinjing Ni, Shuxian Li, Donghua Wen, Jun Li, Haihan Xiao, Ping He, Hong-Yu Ou, Jing Tao, Qihui Teng, Jie Lu, Wenjuan Wu, Yu-Feng Yao |
المصدر: | PLoS Pathogens, Vol 13, Iss 3, p e1006246 (2017) |
بيانات النشر: | Public Library of Science (PLoS), 2017. |
سنة النشر: | 2017 |
المجموعة: | LCC:Immunologic diseases. Allergy LCC:Biology (General) |
مصطلحات موضوعية: | Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5 |
الوصف: | Enterohemorrhagic Escherichia coli (EHEC) is one major type of contagious and foodborne pathogens. The type VI secretion system (T6SS) has been shown to be involved in the bacterial pathogenicity and bacteria-bacteria competition. Here, we show that EHEC could secrete a novel effector KatN, a Mn-containing catalase, in a T6SS-dependent manner. Expression of katN is promoted by RpoS and OxyR and repressed by H-NS, and katN contributes to bacterial growth under oxidative stress in vitro. KatN could be secreted into host cell cytosol after EHEC is phagocytized by macrophage, which leads to decreased level of intracellular reactive oxygen species (ROS) and facilitates the intramacrophage survival of EHEC. Finally, animal model results show that the deletion mutant of T6SS was attenuated in virulence compared with the wild type strain, while the deletion mutant of katN had comparable virulence to the wild type strain. Taken together, our findings suggest that EHEC could sense oxidative stress in phagosome and decrease the host cell ROS by secreting catalase KatN to facilitate its survival in the host cells. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1553-7366 1553-7374 |
Relation: | http://europepmc.org/articles/PMC5363993?pdf=render; https://doaj.org/toc/1553-7366; https://doaj.org/toc/1553-7374 |
DOI: | 10.1371/journal.ppat.1006246 |
URL الوصول: | https://doaj.org/article/58a1d92983394b479790f4e7f08fc5e9 |
رقم الأكسشن: | edsdoj.58a1d92983394b479790f4e7f08fc5e9 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 15537366 15537374 |
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DOI: | 10.1371/journal.ppat.1006246 |