دورية أكاديمية
Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells
العنوان: | Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells |
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المؤلفون: | Yue Zhou, Shuhao Zhang, Yurong Jia, Xi Wang, Yuning Liu, Haolin Zhang, Zhengrong Yuan, Yingying Han, Qiang Weng |
المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 10, p 5155 (2024) |
بيانات النشر: | MDPI AG, 2024. |
سنة النشر: | 2024 |
المجموعة: | LCC:Biology (General) LCC:Chemistry |
مصطلحات موضوعية: | FSH, adiponectin, AdipoR1, AdipoR2, GLUTs, ovarian granulosa cells, Biology (General), QH301-705.5, Chemistry, QD1-999 |
الوصف: | Adiponectin is an important adipokine involved in glucose and lipid metabolism, but its secretion and potential role in regulating glucose utilization during ovarian development remains unclear. This study aims to investigate the mechanism and effects of follicle-stimulating hormones (FSHs) on adiponectin secretion and its following impact on glucose transport in the granulosa cells of rat ovaries. A range of experimental techniques were utilized to test our research, including immunoblotting, immunohistochemistry, immunofluorescence, ELISA, histological staining, real-time quantitative PCR, and transcriptome analysis. The immunohistochemistry results indicated that adiponectin was primarily located in the granulosa cells of rat ovaries. In primary granulosa cells cultured in vitro, both Western blot and immunofluorescence assays demonstrated that FSH significantly induced adiponectin secretion within 2 h of incubation, primarily via the PKA signaling pathway rather than the PI3K/AKT pathway. Concurrently, the addition of the AdipoR1/AdipoR2 dual agonist AdipoRon to the culture medium significantly stimulated the protein expression of GLUT1 in rat granulosa cells, resulting in enhanced glucose absorption. Consistent with these in vitro findings, rats injected with eCG (which shares structural and functional similarities with FSH) exhibited significantly increased adiponectin levels in both the ovaries and blood. Moreover, there was a notable elevation in mRNA and protein levels of AdipoRs and GLUTs following eCG administration. Transcriptomic analysis further revealed a positive correlation between the expression of the intraovarian adiponectin system and glucose transporter. The present study represents a novel investigation, demonstrating that FSH stimulates adiponectin secretion in ovarian granulosa cells through the PKA signaling pathway. This mechanism potentially influences glucose transport (GLUT1) and utilization within the ovaries. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1422-0067 1661-6596 |
Relation: | https://www.mdpi.com/1422-0067/25/10/5155; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
DOI: | 10.3390/ijms25105155 |
URL الوصول: | https://doaj.org/article/58abfdae9940433397a256ec858003ba |
رقم الأكسشن: | edsdoj.58abfdae9940433397a256ec858003ba |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 14220067 16616596 |
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DOI: | 10.3390/ijms25105155 |