دورية أكاديمية
Active site diversification of P450cam with indole generates catalysts for benzylic oxidation reactions
| العنوان: | Active site diversification of P450cam with indole generates catalysts for benzylic oxidation reactions |
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| المؤلفون: | Paul P. Kelly, Anja Eichler, Susanne Herter, David C. Kranz, Nicholas J. Turner, Sabine L. Flitsch |
| المصدر: | Beilstein Journal of Organic Chemistry, Vol 11, Iss 1, Pp 1713-1720 (2015) |
| بيانات النشر: | Beilstein-Institut, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Science LCC:Organic chemistry |
| مصطلحات موضوعية: | active site mutagenesis, biotransformation, C–H activation, cytochrome P450cam monooxygenase, hydroxylation, Science, Organic chemistry, QD241-441 |
| الوصف: | Cytochrome P450 monooxygenases are useful biocatalysts for C–H activation, and there is a need to expand the range of these enzymes beyond what is naturally available. A panel of 93 variants of active self-sufficient P450cam[Tyr96Phe]-RhFRed fusion enzymes with a broad diversity in active site amino acids was developed by screening a large mutant library of 16,500 clones using a simple, highly sensitive colony-based colorimetric screen against indole. These mutants showed distinct fingerprints of activity not only when screened in oxidations of substituted indoles but also for unrelated oxidations such as benzylic hydroxylations. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1860-5397 |
| Relation: | https://doaj.org/toc/1860-5397 |
| DOI: | 10.3762/bjoc.11.186 |
| URL الوصول: | https://doaj.org/article/58d07635f15e406e8cb5f2f4de404bf0 |
| رقم الأكسشن: | edsdoj.58d07635f15e406e8cb5f2f4de404bf0 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 18605397 |
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| DOI: | 10.3762/bjoc.11.186 |