دورية أكاديمية
ROP: dumpster diving in RNA-sequencing to find the source of 1 trillion reads across diverse adult human tissues
| العنوان: | ROP: dumpster diving in RNA-sequencing to find the source of 1 trillion reads across diverse adult human tissues |
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| المؤلفون: | Serghei Mangul, Harry Taegyun Yang, Nicolas Strauli, Franziska Gruhl, Hagit T. Porath, Kevin Hsieh, Linus Chen, Timothy Daley, Stephanie Christenson, Agata Wesolowska-Andersen, Roberto Spreafico, Cydney Rios, Celeste Eng, Andrew D. Smith, Ryan D. Hernandez, Roel A. Ophoff, Jose Rodriguez Santana, Erez Y. Levanon, Prescott G. Woodruff, Esteban Burchard, Max A. Seibold, Sagiv Shifman, Eleazar Eskin, Noah Zaitlen |
| المصدر: | Genome Biology, Vol 19, Iss 1, Pp 1-12 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biology (General) LCC:Genetics |
| مصطلحات موضوعية: | Biology (General), QH301-705.5, Genetics, QH426-470 |
| الوصف: | Abstract High-throughput RNA-sequencing (RNA-seq) technologies provide an unprecedented opportunity to explore the individual transcriptome. Unmapped reads are a large and often overlooked output of standard RNA-seq analyses. Here, we present Read Origin Protocol (ROP), a tool for discovering the source of all reads originating from complex RNA molecules. We apply ROP to samples across 2630 individuals from 54 diverse human tissues. Our approach can account for 99.9% of 1 trillion reads of various read length. Additionally, we use ROP to investigate the functional mechanisms underlying connections between the immune system, microbiome, and disease. ROP is freely available at https://github.com/smangul1/rop/wiki. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1474-760X 78099609 |
| Relation: | http://link.springer.com/article/10.1186/s13059-018-1403-7; https://doaj.org/toc/1474-760X |
| DOI: | 10.1186/s13059-018-1403-7 |
| URL الوصول: | https://doaj.org/article/d5953314c54a44c1a89d0b7809960987 |
| رقم الأكسشن: | edsdoj.5953314c54a44c1a89d0b7809960987 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1474760X 78099609 |
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| DOI: | 10.1186/s13059-018-1403-7 |