دورية أكاديمية

Crocodile blood supplementation protects vascular function in diabetic mice

التفاصيل البيبلوغرافية
العنوان: Crocodile blood supplementation protects vascular function in diabetic mice
المؤلفون: Chui Yiu Bamboo Chook, Francis M. Chen, Gary Tse, Fung Ping Leung, Wing Tak Wong
المصدر: Food Production, Processing and Nutrition, Vol 3, Iss 1, Pp 1-13 (2021)
بيانات النشر: BMC, 2021.
سنة النشر: 2021
المجموعة: LCC:Nutrition. Foods and food supply
LCC:Food processing and manufacture
مصطلحات موضوعية: Crocodile blood, Diabetes mellitus, Vascular endothelial function, Anti-oxidative, Anti-inflammatory, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456
الوصف: Abstract Cardiovascular disease is a major cause of mortality in diabetic patients due to the heightened oxidative stress and pro-inflammatory state in vascular tissues. Effective approaches targeting cardiovascular health for diabetic patients are urgently needed. Crocodile blood, an emerging dietary supplement, was suggested to have anti-oxidative and anti-inflammatory effects in vitro, which have yet to be proven in animal models. This study thereby aimed to evaluate whether crocodile blood can protect vascular function in diabetic mice against oxidation and inflammation. Diabetic db/db mice and their counterparts db/m + mice were treated daily with crocodile blood soluble fraction (CBSF) or vehicle via oral gavage for 4 weeks before their aortae were harvested for endothelium-dependent relaxation (EDR) quantification using wire myograph, which is a well-established functional study for vascular function indication. Organ culture experiments culturing mouse aortae from C57BL/6 J mice with or without IL-1β and CBSF were done to evaluate the direct effect of CBSF on endothelial function. Reactive oxygen species (ROS) levels in mouse aortae were assessed by dihydroethidium (DHE) staining with inflammatory markers in endothelial cells quantified by quantitative polymerase chain reaction (qPCR). CBSF significantly improved deteriorated EDR in db/db diabetic mice through both diet supplementation and direct culture, with suppression of ROS level in mouse aortae. CBSF also maintained EDR and reduced ROS levels in mouse aortae against the presence of pro-inflammatory IL-1β. Under the pro-inflammatory state induced by IL-1β, gene expressions of inflammatory cytokines were downregulated, while the protective transcripts UCP2 and SIRT6 were upregulated in endothelial cells. Our study suggests a novel beneficial effect of crocodile blood on vascular function in diabetic mice and that supplementation of diet with crocodile blood may act as a complementary approach to protect against vascular diseases through anti-oxidation and anti-inflammation in diabetic patients. Graphical abstract
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2661-8974
Relation: https://doaj.org/toc/2661-8974
DOI: 10.1186/s43014-021-00066-w
URL الوصول: https://doaj.org/article/599086c9472d4b8fb2a899e324129072
رقم الأكسشن: edsdoj.599086c9472d4b8fb2a899e324129072
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:26618974
DOI:10.1186/s43014-021-00066-w