Pesticide usage alters plant growth, and development and disturbs native soil microbiome. The present study was carried out to evaluate the effect of Chlorpyrifos (CP-20% EC) on the growth and development of soybean (Glycine max), soil fertility, and soil microbiome. We conducted a pot experiment to assess the impact of different concentrations of chlorpyrifos; recommended dose (RD) and higher dose rates (2× and 4× RD). Chlorpyrifos application significantly altered the growth parameters in soybean (p