دورية أكاديمية
The correlation between the length of repetitive domain and mechanical properties of the recombinant flagelliform spidroin
| العنوان: | The correlation between the length of repetitive domain and mechanical properties of the recombinant flagelliform spidroin |
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| المؤلفون: | Xue Li, Chang-Hua Shi, Chuan-Long Tang, Yu-Ming Cai, Qing Meng |
| المصدر: | Biology Open, Vol 6, Iss 3, Pp 333-339 (2017) |
| بيانات النشر: | The Company of Biologists, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | Flagelliform silk, Wet-spinning, Synthetic fiber, Mechanical properties, Fiber structure, Science, Biology (General), QH301-705.5 |
| الوصف: | Spider silk is an attractive biopolymer with numerous potential applications due to its remarkable characteristics. Among the six categories of spider silks, flagelliform (Flag) spider silk possesses longer and more repetitive core domains than others, therefore performing the highest extensibility. To investigate the correlation between the recombinant spidroin size and the synthetic fiber properties, four recombinant proteins with different sizes [N-Scn-C (n=1-4)] were constructed and expressed using IMPACT system. Subsequently, different recombinant spidroins were spun into fibers through wet-spinning via a custom-made continuous post-drawing device. Mechanical tests of the synthetic fibers with four parameters (maximum stress, maximum extension, Young's modulus and toughness) demonstrated that the extensibility of the fibers showed a positive correlation with spidroin size, consequently resulting in the extensibility of N-Sc4-C fiber ranked the highest (58.76%) among four fibers. Raman data revealed the relationship between secondary structure content and mechanical properties. The data here provide a deeper insight into the relationship between the function and structure of Flag silk for future design of artificial fibers. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2046-6390 |
| Relation: | http://bio.biologists.org/content/6/3/333; https://doaj.org/toc/2046-6390 |
| DOI: | 10.1242/bio.022665 |
| URL الوصول: | https://doaj.org/article/dc5a70deafc8483992ba079f7a307f26 |
| رقم الأكسشن: | edsdoj.5a70deafc8483992ba079f7a307f26 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20466390 |
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| DOI: | 10.1242/bio.022665 |