دورية أكاديمية
Therapeutic Potential of Vitamin D and Curcumin in an Model of Alzheimer Disease
| العنوان: | Therapeutic Potential of Vitamin D and Curcumin in an Model of Alzheimer Disease |
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| المؤلفون: | Abir Abdullah Alamro, Ebtesam Atiah Alsulami, Moudhi Almutlaq, Amani Alghamedi, Majed Alokail, Samina Hyder Haq |
| المصدر: | Journal of Central Nervous System Disease, Vol 12 (2020) |
| بيانات النشر: | SAGE Publishing, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Neurology. Diseases of the nervous system |
| مصطلحات موضوعية: | Neurology. Diseases of the nervous system, RC346-429 |
| الوصف: | Background: Alzheimer disease is a progressive neurodegenerative disease, affecting a very high proportion of the aging population. Several studies have demonstrated that one of the main contributors to this disease is oxidative stress (OS), which causes peroxidation of protein, lipids, and DNA resulting in the formation of advanced glycosylated end products (AGE) in the brain tissues. These AGE are usually associated with the amyloid β (Aβ), which could further aggravate its toxicity and its clearance. Antioxidants counteract the deterioration caused by OS. Objective: We aimed to evaluate the effect of vitamin D3 and curcumin on primary cortical neuronal cultures exposed to Aβ 1-42 toxicity for different time periods. Methods: Primary cortical neuronal cultures were set up and exposed to Aβ 1-42 for up to 72 hours. Cell viability was studied by 3[4,5-dimethylthiazole-2-yl]-2,5-dipheyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assay. Biochemical assays for OS such as lipid peroxidation, reduced Glutathione(GSH), Glutathione S-transferase (GST), catalase, and superoxide dismutase (SOD) were conducted. Sandwich enzyme-linked immunosorbent assay (ELISA) was used to study the neurotrophic growth factor (NGF) expression. Results: Treatments with Aβ 1-42 caused an elevation in lipid peroxidation products, which were ameliorated in the presence of vitamin D3 and curcumin. Both enzymatic (GST, catalase, and SOD) and nonenzymatic antioxidants (reduced GSH) were raised significantly in the presence of vitamin D3 and curcumin, which resulted in the better recovery of neuronal cells from Aβ 1-42 treatment. Treatment with vitamin D3 and curcumin also resulted in the upregulation of NGF levels. Conclusions: This study suggests that vitamin D3 and curcumin can be a promising natural therapy for the treatment of Alzheimer disease. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1179-5735 11795735 |
| Relation: | https://doaj.org/toc/1179-5735 |
| DOI: | 10.1177/1179573520924311 |
| URL الوصول: | https://doaj.org/article/e5ac6cf58f7749f6bcd866f4c6decdd5 |
| رقم الأكسشن: | edsdoj.5ac6cf58f7749f6bcd866f4c6decdd5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 11795735 |
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| DOI: | 10.1177/1179573520924311 |